Bacterial extracellular vesicles (BEVs) possess features that make them well suited for the delivery of therapeutics and vaccines. This chapter describes methods for engineering the commensal human intestinal bacterium Bacteroides thetaiotaomicron (Bt) to produce BEVs carrying vaccine antigens and accompanying methods for isolating and purifying BEVs for mucosal vaccination regimens.
Keywords: Bacterial extracellular vesicles; Bacteroides thetaiotaomicron; Crossflow ultrafiltration; Immunization; Vaccines.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.