The impact of the cavitary corpus luteum on the blood progesterone concentration and pregnancy rate of embryo recipient heifers

Abstract

In cattle there are two types of the corpus luteum (CL): homogeneous (CL_hom) and cavitary (CL_cav). Although they are considered equal in their hormonal activity, the function of the CL_cav is questioned by many veterinarians. In consequence, females with the CL_cav are considered less valuable for assisted reproductive techniques such as embryo transfer (ET), where recipients with the CL_hom are preferred. The aim of our study was to compare the two types of CLs regarding morphological endpoints, serum progesterone (P4) concentrations and final pregnancy rate (PR) in recipient heifers after ET. The morphological type of the CL and the final PR after ET of 432 Holstein Friesian heifers were analyzed. Oestrus was synchronized with two i.m. inj. of 0.5 μg cloprostenol 14 days apart. The ET took place on the day 7 of the estrous cycle, only animals with visible oestrum were chosen for the procedure. Clinical and transrectal US examinations of ovaries were performed on the day of ET. The presence of the CL_hom or CL_cav was determined, and the CL diameter and cross-sectional area were measured. If present, measurements of the cavities were also taken. Only embryos recovered immediately prior to the ET at the morula or blastocyst stage were transferred to the randomly chosen recipient that underwent initial selection regardless of the CL morphology. Additionally, from randomly selected heifers (N = 53, CL_hom = 33; CL_cav = 20) blood samples for serum P4 concentration analysis were collected. Pregnancy was determined by transrectal palpation 2 months after ET. The medium-sized CL_cav was larger in diameter (P < 0.001) and cross-sectional area (P < 0.01) than the CL_hom (mean ± SD) - 23.29 ± 3.6 mm and 419.57 ± 135.01 mm² compared to 21.87 ± 3.57 mm and 384.73 ± 145.46 mm², respectively. The mean diameter and cross-sectional area of the cavity were 10.2 ± 4.36 mm and 97.59 ± 71.13 mm², respectively. The volume of both types of CLs was similar (P = 0.3). The mean serum P4 concentration was 8.84 ng/ml, higher (P < 0.0001) for females with the CL_cav (11.31 ng/ml) than for those with the CL_hom (7.15 ng/ml). The PR was 36.1%, higher (P < 0.05) for recipients with the CL_cav (47.7%) compared to the CL_hom (29.9%). The presence of a CL_cav in the recipient heifers did not negatively affect the potential of the CL to maintain pregnancy. On the contrary, the CL_cav may give the embryo better chances of surviving the time of pregnancy recognition and in consequence, may have a positive effect on PR in heifers.

Keywords: Cattle; Cavity; Corpus luteum; Embryo transfer; Heifers.