Generation of a Spiral Ganglion Neuron Degeneration Mouse Model

Front Cell Dev Biol. 2021 Oct 27:9:761847. doi: 10.3389/fcell.2021.761847. eCollection 2021.

Abstract

Spiral ganglion neurons (SGNs) can be injured by a wide variety of insults. However, there still is a lack of degeneration models to specifically damage the SGNs without disturbing other types of cells in the inner ear. This study aims to generate an SGN-specific damage model using the Cre-LoxP transgenic mouse strains. The Cre-inducible diphtheria toxin receptor (iDTR+/+ ) knock-in mouse strain was crossed with a mouse strain with Cre activity specific to neurons (Nefl CreER/CreER ). Expression of the Cre-recombinase activity was evaluated using the reporter mouse strain Ai9 at pre-hearing, hearing onset, and post-hearing stages. Accordingly, heterozygous Nefl CreER/+;iDTR+/- mice were treated with tamoxifen on postnatal days 1-5 (P1-5), followed by diphtheria toxin (DT) or vehicle injection on P7, P14, and P21 to evaluate the SGN loss. Robust tamoxifen-induced Cre-mediated Ai9 tdTomato fluorescence was observed in the SGN area of heterozygous Nefl CreER/+;Ai9+/- mice treated with tamoxifen, whereas vehicle-treated heterozygote mice did not show tdTomato fluorescence. Compared to vehicle-treated Nefl CreER/+;iDTR+/- mice, DT-treated Nefl CreER/+;iDTR+/- mice showed significant auditory brainstem response (ABR) threshold shifts and SGN cell loss. Hair cell count and functional study did not show significant changes. These results demonstrate that the Nefl CreER/CreER mouse strain exhibits inducible SGN-specific Cre activity in the inner ear, which may serve as a valuable SGN damage model for regeneration research of the inner ear.

Keywords: Cre-LoxP; auditory brainstem response; degeneration; iDTR; neurofilament; spiral ganglion.