Preclinical Application of Conditional Reprogramming Culture System for Laryngeal and Hypopharyngeal Carcinoma

Yanbo Dong; Jian Wang; Wei Ji; Mengzhu Zheng; Peng Wang; Liangfa Liu; Shanhu Li

doi:10.3389/fcell.2021.744969

Preclinical Application of Conditional Reprogramming Culture System for Laryngeal and Hypopharyngeal Carcinoma

Front Cell Dev Biol. 2021 Oct 29:9:744969. doi: 10.3389/fcell.2021.744969. eCollection 2021.

Authors

Yanbo Dong¹, Jian Wang², Wei Ji¹, Mengzhu Zheng², Peng Wang², Liangfa Liu¹, Shanhu Li²

Affiliations

¹ Department of Otolaryngology Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, China.
² Department of Cell Engineering, Beijing Institute of Biotechnology, Beijing, China.

Abstract

Management of laryngeal and hypopharyngeal squamous cell carcinoma (LHSCC) remains highly challenging due to highly variable therapeutic responses. By establishing an in vitro model for LHSCC based on conditional reprogramming (CR), a cell-culture technique, we aim to investigate its potential value on personalized cancer therapies. Herein, a panel of 28 human LHSCC CR cells were established from 50 tumor tissues using the CR method. They retained tumorigenic potential upon xenotransplantation and recapitulated molecular characteristics of LHSCC. Differential responses to anticancer drugs and radiotherapy were detected in vitro. CR cells could be transformed to xenograft and organoid, and they shared comparable drug responses. The clinical drug responses were consistent with in vitro drug responses. Collectively, the patient-derived CR cell model could promisingly be utilized in clinical decision-making and assisted in the selection of personalized therapies for LHSCC.

Keywords: conditional reprogramming; drug sensitivity; head and neck squamous cell carcinoma; in vitro model; personalized treatment.