Protective effect of antioxidant peptides from grass carp scale gelatin on the H2O2-mediated oxidative injured HepG2 cells

Yue-Ming Hu; Su-Zhen Lu; Ya-Si Li; Hui Wang; Yan Shi; Lu Zhang; Zong-Cai Tu

doi:10.1016/j.foodchem.2021.131539

Protective effect of antioxidant peptides from grass carp scale gelatin on the H₂O₂-mediated oxidative injured HepG2 cells

Food Chem. 2022 Mar 30;373(Pt B):131539. doi: 10.1016/j.foodchem.2021.131539. Epub 2021 Nov 8.

Authors

Yue-Ming Hu¹, Su-Zhen Lu¹, Ya-Si Li¹, Hui Wang¹, Yan Shi², Lu Zhang³, Zong-Cai Tu⁴

Affiliations

¹ State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China.
² State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China. Electronic address: shiyan@ncu.edu.cn.
³ National R&D Center of Freshwater Fish Processing, Jiangxi Normal University, Nanchang 330022, China; Engineering Research Center of Freshwater Fish High-value Utilization of Jiangxi Province, Jiangxi Normal University, Nanchang 330022, China.
⁴ State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China; National R&D Center of Freshwater Fish Processing, Jiangxi Normal University, Nanchang 330022, China; Engineering Research Center of Freshwater Fish High-value Utilization of Jiangxi Province, Jiangxi Normal University, Nanchang 330022, China. Electronic address: tuzc_mail@aliyun.com.

PMID: 34776311
DOI: 10.1016/j.foodchem.2021.131539

Abstract

The protective effect of antioxidant peptides from grass carp scale gelatin on hydrogen peroxide (H₂O₂)-mediated oxidative injured HepG2 cells was investigated, and the protective mechanism as well as peptide structure were studied. Pretreated with grass carp scale gelatin hydrolysates (GSGH) for 24 h significantly increased the survival rates and decreased the apoptosis rates of H₂O₂-mediated oxidative injured HepG2 cells. The increase in SOD, CAT and GPX activities, reduce in ROS level and MDA content, and weaken in damage on cell membrane and DNA could be responsible for the protective effect of GSGH on H₂O₂-mediated oxidative injured HepG2 cells. Peptide sequences of GSGH were analyzed by LC-ESI-Q-Orbitrap-MS/MS, and results showed that most of them were low molecular weight peptide at 358-986 Da. Synergistic effect existed among antioxidant peptides and contributed to the strong antioxidant activities of GSGH.

Keywords: Antioxidant peptide; Fish scale gelatin; Oxidative injured HepG2 cells; Protective effect.

MeSH terms

Animals
Antioxidants* / metabolism
Antioxidants* / pharmacology
Carps* / metabolism
Cell Survival
Gelatin
Hep G2 Cells
Humans
Hydrogen Peroxide
Oxidative Stress
Peptides / pharmacology
Tandem Mass Spectrometry

Substances

Antioxidants
Peptides
Gelatin
Hydrogen Peroxide