bZIP72 promotes submerged rice seed germination and coleoptile elongation by activating ADH1

Shuang Wang; Wanning Liu; Yong He; Tosin Victor Adegoke; Jiezheng Ying; Xiaohong Tong; Zhiyong Li; Liqun Tang; Huimei Wang; Jian Zhang; Zhihong Tian; Yifeng Wang

doi:10.1016/j.plaphy.2021.11.005

bZIP72 promotes submerged rice seed germination and coleoptile elongation by activating ADH1

Plant Physiol Biochem. 2021 Dec:169:112-118. doi: 10.1016/j.plaphy.2021.11.005. Epub 2021 Nov 6.

Authors

Affiliations

¹ Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, Hubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland, College of Life Science, Yangtze University, Jingzhou, 434025, China.
² State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 311400, China.
³ State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 311400, China; State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, LinAn, 311300, China.
⁴ State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 311400, China. Electronic address: zhangjian@caas.cn.
⁵ Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, Hubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland, College of Life Science, Yangtze University, Jingzhou, 434025, China. Electronic address: zhtian@yangtzeu.edu.cn.
⁶ State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 311400, China. Electronic address: wangyifeng@caas.cn.

PMID: 34775177
DOI: 10.1016/j.plaphy.2021.11.005

Abstract

Seed germination and coleoptile elongation in response to flooding stress is an important trait for the direct seeding of rice. However, the genes regulating this process and the underlying mechanisms are little understood. In this study, bZIP72 was identified as a positive regulator of seed germination under submergence. Transcription of bZIP72 was submergence induced. Over-expression of bZIP72 enhanced submerged seed germination and coleoptile elongation, while bzip72 mutants exhibited the opposite tendency. Using biochemical interaction assays, we showed that bZIP72 directly binds to the promoter of alcohol dehydrogenase 1 (ADH1), enhances its activity, and subsequently produces more NAD⁺, NADH and ATP involved in the alcoholic fermentation and glycolysis pathway, ultimately providing necessary energy reserves thus conferring tolerance to submergence. In summary, this research provides novel insights into bZIP72 participation in submerged rice seed germination and coleoptile elongation.

Keywords: ADH1; Coleoptile elongation; Rice (Oryza sativa L.); Seed germination; Submergence; bZIP72.

MeSH terms

Cotyledon
Germination
Oryza* / genetics
Seeds