Protein sulfenylation contributes to oxidative burst-triggered responses during the interaction between Botrytis cinerea and Nicotiana benthamiana

Abstract

Reactive oxygen species (ROS) play a crucial role as signaling molecules in plant responses to pathogen infection. It is highly reactive with cellular components such as DNA, lipids and proteins, thereby leading to serious oxidative damages. Cysteine residues are sensitive targets of ROS in a post-translational modification known as sulfenylation. However, during plant-pathogen interaction, it is still unclear which specific proteins can be oxidized by ROS and undergo sulfenic modification to regulate the interaction process. Here, we observed a biphasic production of ROS in Nicotiana benthamiana after inoculation with Botrytis cinerea. RT-qPCR results showed that the biphasic increase in ROS production was closely related to the expression of NbRbohA, NbRbohB and NbRbohC. Furthermore, a ROS-dependent sulfenome analysis was performed and finally 183 differentially sulfenylated proteins were identified. Their post-translational sulfenylation modification in response to B. cinerea infection was further confirmed by western blot and mass spectrometry analysis. Virus-induced gene silencing of those genes encoding sulfenylated proteins resulted in reduced resistance to B. cinerea. Taken together, our data demonstrate that B. cinerea infection induces ROS burst in N. benthamiana, which triggers protein sulfenylation to ensure the transduction of ROS signals and further function in plant-pathogen interaction. SIGNIFICANCE: Reactive oxygen species (ROS) induced by Botrytis cinerea infection trigger changes in cellular redox status through protein sulfenylation to be involved in plant-pathogen interaction.

Keywords: Botrytis cinerea; Nicotiana benthamiana; Oxidative stress; Protein sulfenylation; Reactive oxygen species; Sulfenome.