The quantitative proteome atlas of a model cyanobacterium

Jinlong Wang; Xiahe Huang; Haitao Ge; Yan Wang; Weiyang Chen; Limin Zheng; Chengcheng Huang; Haomeng Yang; Lingyu Li; Na Sui; Yu Wang; Yuanya Zhang; Dandan Lu; Longfa Fang; Wu Xu; Yuqiang Jiang; Fang Huang; Yingchun Wang

doi:10.1016/j.jgg.2021.09.007

The quantitative proteome atlas of a model cyanobacterium

J Genet Genomics. 2022 Feb;49(2):96-108. doi: 10.1016/j.jgg.2021.09.007. Epub 2021 Nov 11.

Authors

Jinlong Wang¹, Xiahe Huang², Haitao Ge², Yan Wang³, Weiyang Chen³, Limin Zheng³, Chengcheng Huang³, Haomeng Yang⁴, Lingyu Li⁴, Na Sui⁵, Yu Wang², Yuanya Zhang², Dandan Lu⁶, Longfa Fang³, Wu Xu⁷, Yuqiang Jiang³, Fang Huang⁸, Yingchun Wang⁹

Affiliations

¹ State Key Laboratory of Molecular Developmental Biology, The Innovative Academy of Seed Design, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, Zhejiang 310024, China.
² State Key Laboratory of Molecular Developmental Biology, The Innovative Academy of Seed Design, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
³ State Key Laboratory of Molecular Developmental Biology, The Innovative Academy of Seed Design, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
⁴ Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Bejing 100093, China.
⁵ Shandong Provincial Key Laboratory of Plant Stress, College of Life Science, Shandong Normal University, Jinan, Shandong 250014, China.
⁶ State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng, Henan 475004, China.
⁷ Department of Chemistry, University of Louisiana at Lafayette, Lafayette, LA 70504, USA.
⁸ Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Bejing 100093, China. Electronic address: fhuang@ibcas.ac.cn.
⁹ State Key Laboratory of Molecular Developmental Biology, The Innovative Academy of Seed Design, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: ycwang@genetics.ac.cn.

PMID: 34775074
DOI: 10.1016/j.jgg.2021.09.007

Abstract

Cyanobacteria are a group of oxygenic photosynthetic bacteria with great potentials in biotechnological applications and advantages as models for photosynthesis research. The subcellular localizations of the majority of proteins in any cyanobacteria remain undetermined, representing a major challenge in using cyanobacteria for both basic and industrial researches. Here, using label-free quantitative proteomics, we map 2027 proteins of Synechocystis sp. PCC6803, a model cyanobacterium, to different subcellular compartments and generate a proteome atlas with such information. The atlas leads to numerous unexpected but important findings, including the predominant localization of the histidine kinases Hik33 and Hik27 on the thylakoid but not the plasma membrane. Such information completely changes the concept regarding how the two kinases are activated. Together, the atlas provides subcellular localization information for nearly 60% proteome of a model cyanobacterium, and will serve as an important resource for the cyanobacterial research community.

Keywords: Cyanobacteria; Membranes; Proteome atlas; Proteomics; Synechocystis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Proteome* / genetics
Proteome* / metabolism
Synechocystis* / genetics
Synechocystis* / metabolism

Substances

Bacterial Proteins
Proteome