The Role of RAD6B and PEDF in Retinal Degeneration

Qiang Ye; Jiaqi Wang; Xiangwen Liu; Zihua Liu; LuoSong BaZong; Jinhai Ma; Rong Shen; Weichun Ye; Wenfang Zhang; Degui Wang

doi:10.1016/j.neuroscience.2021.11.010

The Role of RAD6B and PEDF in Retinal Degeneration

Neuroscience. 2022 Jan 1:480:19-31. doi: 10.1016/j.neuroscience.2021.11.010. Epub 2021 Nov 11.

Authors

Qiang Ye¹, Jiaqi Wang², Xiangwen Liu², Zihua Liu², LuoSong BaZong³, Jinhai Ma³, Rong Shen⁴, Weichun Ye⁵, Wenfang Zhang⁶, Degui Wang⁷

Affiliations

¹ Institute of Human Anatomy and Histoembryology, Basic Medical College, Lanzhou University, 199 Donggang West Road, Lanzhou 730000, China; Department of Ophthalmology, The Second Hospital of Lanzhou University, 82 Cuiying Door, Lanzhou 730000, China.
² Institute of Human Anatomy and Histoembryology, Basic Medical College, Lanzhou University, 199 Donggang West Road, Lanzhou 730000, China.
³ Department of Ophthalmology, The Second Hospital of Lanzhou University, 82 Cuiying Door, Lanzhou 730000, China.
⁴ Institute of Human Anatomy and Histoembryology, Basic Medical College, Lanzhou University, 199 Donggang West Road, Lanzhou 730000, China. Electronic address: shenr@lzu.edu.cn.
⁵ State Key Laboratory of Applied Organic Chemistry and Department of Chemistry, Lanzhou University, Lanzhou 730000, China. Electronic address: yewch@lzu.edu.cn.
⁶ Department of Ophthalmology, The Second Hospital of Lanzhou University, 82 Cuiying Door, Lanzhou 730000, China. Electronic address: zhwenf888@163.com.
⁷ Institute of Human Anatomy and Histoembryology, Basic Medical College, Lanzhou University, 199 Donggang West Road, Lanzhou 730000, China. Electronic address: wangdegui@lzu.edu.cn.

PMID: 34774969
DOI: 10.1016/j.neuroscience.2021.11.010

Abstract

RAD6B is an E2 ubiquitin-conjugating enzyme, playing an important role in DNA damage repair, gene expression, senescence, apoptosis and protein degradation. However, the specific mechanism between ubiquitin and retinal degeneration requires more investigation. Pigment epithelium-derived factor (PEDF) has a potent neurotrophic effect on the retina, protecting retinal neurons and photoreceptors from cell death caused by pathological damage. In this study, we found that loss of RAD6B leads to retinal degeneration in mice, especially in old age. Affymetrix microarray analysis showed that the PEDF signal was changed in RAD6B deficient groups. The expression of γ-H2AX, β-Gal, P53, Caspase-3, P21 and P16 was increased significantly in retinas of RAD6B knockout (KO) mice. Our studies suggest that RAD6B and PEDF play an important role in the health of retina, whereas the absence of RAD6B accelerates the degeneration.

Keywords: PEDF; RAD6B; apoptosis; degeneration; retina; senescence.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Eye Proteins / genetics
Mice
Nerve Growth Factors / genetics
Retina
Retinal Degeneration*
Serpins* / genetics

Substances

Eye Proteins
Nerve Growth Factors
Serpins
pigment epithelium-derived factor