Recent advances in enzyme-enhanced immunosensors

Yanna Shao; Huan Zhou; Qingping Wu; Yonghua Xiong; Juan Wang; Yu Ding

doi:10.1016/j.biotechadv.2021.107867

Recent advances in enzyme-enhanced immunosensors

Biotechnol Adv. 2021 Dec:53:107867. doi: 10.1016/j.biotechadv.2021.107867. Epub 2021 Nov 12.

Authors

Yanna Shao¹, Huan Zhou¹, Qingping Wu², Yonghua Xiong³, Juan Wang⁴, Yu Ding⁵

Affiliations

¹ Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China; Department of Food Science and Technology, Institute of Food Safety and Nutrition, College of Science & Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
² Department of Food Science and Technology, Institute of Food Safety and Nutrition, College of Science & Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
³ State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
⁴ College of Food Science, South China Agricultural University, Guangzhou 510432, China.
⁵ Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China; Department of Food Science and Technology, Institute of Food Safety and Nutrition, College of Science & Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China. Electronic address: dingyu@jnu.edu.cn.

PMID: 34774928
DOI: 10.1016/j.biotechadv.2021.107867

Abstract

Among the products for rapid detection in different fields, enzyme-based immunosensors have received considerable attention. Recently, great efforts have been devoted to enhancing the output signals of enzymes through different strategies that can significantly improve the sensitivity of enzyme-based immunosensors for the need of practical applications. In this manuscript, the significance of enzyme-based signal transduction patterns in immunoassay and the central role of enzymes in achieving precise control of reaction systems are systematically described. In view of the rapid development of this field, we classify these strategies based on the combination of immune recognition and enzyme amplification into three categories, namely enzyme-based enhancement strategies, combination of the catalytic amplification of enzymes with other signal amplification methods, and substrate-based enhancement strategies. The current focus and future direction of enzyme-based immunoassays are also discussed. This article is not exhaustive, but focuses on the latest advances in different signal generation methods based on enzyme-initiated catalytic reactions and their applications in the detection field, which could provide an accessible introduction of enzyme-based immunosensors for the community with a view to further improving its application efficiency.

Keywords: Cascade reaction; Enzyme; Immunoassay; Nanozyme; Signal amplification.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Biosensing Techniques*
Immunoassay