A multistep enrichment process with custom growth medium improves resuscitation of chlorine-stressed coliforms from secondary sewage effluents

Jennifer M Mobberley; Scott K Cooley; Mark W Widder; Shelby M Phillips; Angela M Melville; Linda M Brennan; Valerie T Divito; William H van der Schalie; Richard M Ozanich; Janine R Hutchison

doi:10.1016/j.mimet.2021.106364

A multistep enrichment process with custom growth medium improves resuscitation of chlorine-stressed coliforms from secondary sewage effluents

J Microbiol Methods. 2022 Jan:192:106364. doi: 10.1016/j.mimet.2021.106364. Epub 2021 Nov 12.

Affiliations

¹ Chemical and Biological Signatures Group, National Security Directorate, Pacific Northwest National Laboratory, P.O. Box 999, Richland, WA 99352, USA.
² Statistical Modeling and Experiment Design Group, National Security Directorate, Pacific Northwest National Laboratory, P.O. Box 999, Richland, WA 99352, USA.
³ Experimental Therapeutics Branch, Walter Reed Army Institute of Research, Fort Detrick, MD 21702-5000, USA.
⁴ U.S. Army Medical Research and Development Command, Military Operational Medicine Research Program, Fort Detrick, MD 21702-5000, USA.
⁵ Experimental Therapeutics Branch, Walter Reed Army Institute of Research, Fort Detrick, MD 21702-5000, USA; General Dynamics Information Technology, Falls Church, VA 22042, USA.
⁶ Chemical and Biological Signatures Group, National Security Directorate, Pacific Northwest National Laboratory, P.O. Box 999, Richland, WA 99352, USA. Electronic address: janine.hutchison@pnnl.gov.

PMID: 34774876
DOI: 10.1016/j.mimet.2021.106364

Abstract

Resuscitation and detection of stressed total coliforms in chlorinated water samples is needed to assess and prevent health effects from adverse exposure. In this study, we report that the addition of a growth enhancer mix consisting of trehalose, sodium pyruvate, magnesium chloride, and 1× trace mineral supplement improved growth of microorganisms from chlorinated secondary effluent in the base medium with Colilert-18. Improving growth of chlorine stressed microorganisms from secondary effluent is crucial to decreased detection time from 18 to 8 h.

Keywords: Chlorination; Chlorine-stressed; EPA alternate test procedure; Escherichia coli; Secondary sewage effluent; Total coliforms.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Bacterial Load / methods*
Chlorine / toxicity*
Culture Media / chemistry*
Environmental Monitoring / methods*
Escherichia coli / growth & development*
Fluoridation
Magnesium Chloride / metabolism
Pyruvates / metabolism
Sewage / microbiology*
Trehalose / metabolism
Water Microbiology

Substances

Culture Media
Pyruvates
Sewage
Magnesium Chloride
Chlorine
Trehalose