Forensic application of a novel MPS-based panel (90 STRs and 100 SNPs) in a non-exclusion parentage case with three autosomal STRs incompatibilities

Jia-Zi Wu; Ling-Xiang Wang; Xing-Yi Yang; Dong-Hua Pan; Xiao-Yu Lu; Chang-Hui Liu; Xiao-Long Han; Hong Liu; Mei-Sen Shi; Chao Liu; Shao-Qing Wen

doi:10.1016/j.legalmed.2021.101987

Forensic application of a novel MPS-based panel (90 STRs and 100 SNPs) in a non-exclusion parentage case with three autosomal STRs incompatibilities

Leg Med (Tokyo). 2022 Feb:54:101987. doi: 10.1016/j.legalmed.2021.101987. Epub 2021 Nov 3.

Authors

Jia-Zi Wu¹, Ling-Xiang Wang², Xing-Yi Yang³, Dong-Hua Pan⁴, Xiao-Yu Lu², Chang-Hui Liu³, Xiao-Long Han³, Hong Liu³, Mei-Sen Shi⁵, Chao Liu⁶, Shao-Qing Wen⁷

Affiliations

¹ MOE Key Laboratory of Contemporary Anthropology, Department of Anthropology and Human Genetics, School of Life Sciences, Fudan University, Shanghai 200438, China; Institute of Archaeological Science, Fudan University, Shanghai 200433, China.
² Institute of Archaeological Science, Fudan University, Shanghai 200433, China; Deepreads Biotech, Guangzhou 510663, China.
³ Guangzhou Forensic Science Institute, Guangzhou 510030, China.
⁴ Forensic Science Centre of Maoming Public Security Department, Guangdong Province, Maoming 525000, China.
⁵ Criminal Justice College of China University of Political Science and Law, Beijing 100088, China. Electronic address: shimeisen2000@163.com.
⁶ Guangzhou Forensic Science Institute, Guangzhou 510030, China. Electronic address: liuchaogzf@163.com.
⁷ MOE Key Laboratory of Contemporary Anthropology, Department of Anthropology and Human Genetics, School of Life Sciences, Fudan University, Shanghai 200438, China; Institute of Archaeological Science, Fudan University, Shanghai 200433, China. Electronic address: wenshaoqing@fudan.edu.cn.

PMID: 34768042
DOI: 10.1016/j.legalmed.2021.101987

Abstract

In kinship tests, the investigating of the forensic STRs usually provides decisive information to resolve relationship cases. We describe a parentage case with 3 genetic incompatibilities (D6S1043, D18S51 and D2S1338) between the child and alleged parent. With 90 STR loci and 100 SNP loci, the massively parallel sequencing (MPS)-based genotyping results support the certainty of parentage, and the mismatched alleles were considered to be mutations. MPS can provide additional allele sequence structures that can be used to infer the origins of the mutations. SNPs as supplementary markers can provide effective information to give an unequivocal statement of the parentage.

Keywords: Autosomal STRs; MPS; Mutation; Parentage testing; SNPs.

MeSH terms

Child
DNA Fingerprinting*
High-Throughput Nucleotide Sequencing
Humans
Microsatellite Repeats / genetics
Polymorphism, Single Nucleotide* / genetics
Sequence Analysis, DNA