Potential of food waste hydrolysate as an alternative carbon source for microbial oil synthesis

Abstract

Volatile fatty acids (VFAs) have great potential as cheap raw materials in microbial oil synthesis and reducing the cost of substrates is essential for the development of microbial oil biosynthesis. In this study, the food waste hydrolysate and synthetic VFAs media were both used as substrate to synthesis microbial oil. The optimal short-chain VFAs ratio for microbial oil synthesis is 20:5:5 and increasing the proportion of propionic acid is the key to obtaining odd fatty acids. The hydrolysate obtained from food waste under the total solid condition of 2:1 and pH 5 is the most suitable medium for microbial oil synthesis. The biological products obtained from food waste hydrolysate were comparable to synthetic VFAs media, obtaining a 34.02% of lipid content. Results prove that food waste hydrolysate has great potential as the available feedstock for microbial oil synthesis and a promising application value in food waste recycling.

Keywords: Food waste; Hydrolysate; Microbial oil; VFAs; Yeast.