New Tools for Streamlined In Vivo Homing Peptide Identification

Kristina Põšnograjeva; Karlis Pleiko; Maarja Haugas; Tambet Teesalu

doi:10.1007/978-1-0716-1752-6_25

New Tools for Streamlined In Vivo Homing Peptide Identification

Methods Mol Biol. 2022:2383:385-412. doi: 10.1007/978-1-0716-1752-6_25.

Authors

Kristina Põšnograjeva^#¹, Karlis Pleiko^#^{1

2}, Maarja Haugas¹, Tambet Teesalu^{3

4

5}

Affiliations

¹ Laboratory of Precision and Nanomedicine, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.
² Faculty of Medicine, University of Latvia, Riga, Latvia.
³ Laboratory of Precision and Nanomedicine, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia. tambet.teesalu@ut.ee.
⁴ Cancer Research Center, Sanford-Burnham-Prebys Medical Discovery Institute, La Jolla, CA, USA. tambet.teesalu@ut.ee.
⁵ Center for Nanomedicine and Department of Cell, Molecular and Developmental Biology, University of California, Santa Barbara, CA, USA. tambet.teesalu@ut.ee.

^# Contributed equally.

PMID: 34766303
DOI: 10.1007/978-1-0716-1752-6_25

Abstract

In vivo peptide-phage display is an unbiased technique for mapping of the vascular diversity and identification of homing peptides. This chapter is intended to serve as a structured practical guide to execute in vivo T7 phage biopanning and data analysis experiments. We discuss experimental designs and protocols with emphasis on application of high-throughput sequencing-based technologies for streamlined in vivo biopanning and validation of homing peptides.

Keywords: Affinity targeting; High-throughput sequencing; Homing peptide; In vivo phage display; T7 bacteriophage; Vascular ZIP codes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteriophage T7 / genetics
Cell Surface Display Techniques
High-Throughput Nucleotide Sequencing
Peptide Library
Peptides / chemistry*

Substances

Peptide Library
Peptides