Synthesis of Cell-Penetrating Peptide Coated Silica Nanoparticles and Their Physicochemical and Biological Characterization

Isabel Gessner; Annika Klimpel; Ines Neundorf

doi:10.1007/978-1-0716-1752-6_7

Synthesis of Cell-Penetrating Peptide Coated Silica Nanoparticles and Their Physicochemical and Biological Characterization

Methods Mol Biol. 2022:2383:105-117. doi: 10.1007/978-1-0716-1752-6_7.

Authors

Isabel Gessner¹, Annika Klimpel², Ines Neundorf³

Affiliations

¹ Department of Chemistry, Institute of Inorganic Chemistry, University of Cologne, Cologne, Germany.
² Department of Chemistry, Institute for Biochemistry, University of Cologne, Cologne, Germany.
³ Department of Chemistry, Institute for Biochemistry, University of Cologne, Cologne, Germany. ines.neundorf@uni-koeln.de.

PMID: 34766285
DOI: 10.1007/978-1-0716-1752-6_7

Abstract

The surface decoration of nanoparticles with cell-penetrating peptides (CPPs) represents a common technique for intracellular delivery of nanotherapeutics. Conjugate formation can be performed via covalent or non-covalent strategies. Here, we describe on the synthesis of silica nanoparticles, a well-known inorganic drug delivery vehicle type, and their surface modification with cell-penetrating peptides using sC18 and derivatives thereof. Moreover, physicochemical as well as biological characterization methods, including cellular uptake measurements, of particle-peptide conjugates are described.

Keywords: Cell-penetrating peptides; Cellular uptake; Internalization; Nanoparticles; Particle-peptide conjugate; Surface modification.

MeSH terms

Cell-Penetrating Peptides
Drug Delivery Systems
Nanoparticles*
Silicon Dioxide

Substances

Cell-Penetrating Peptides
Silicon Dioxide