With the steady development of metal-organic framework (MOF) materials, this peculiar class of three-dimensional materials has found application prospects in a myriad of areas. The integration of different metals with various categories of ligands engendered a full gamut of frameworks, which of course are supplemented by diversified modification methods. Amongst many metal centers utilized to design and synthesize targeted MOFs, alkali/alkaline earth metal-based MOFs are gaining significant attention because these metal centers can be regarded as human endogenous metals. Numerous studies have shown that alkali/alkaline earth metal MOFs (A/A-E MOFs) tend to have better properties than other metals. This is because A/A-E MOFs offer better biocompatibility, so it is expected to be used in a broader field of biomedicine in the near future. This review mainly introduces the application of A/A-E MOF materials in drug delivery, sensing, and some materials with unique biomedical applications, and elaborates the challenges, obstacles and development of some A/A-E MOF materials in the biomedical field.