YQHX Alleviates H/R-Induced Cardiomyocyte Apoptosis by Downregulating miR-1

Luandie Ge; Yaqi Fan; Lin Fu; Mengjiao Guo; Panxia Cao; Chaojie Peng; Linke Wu; Lihua Han; Hong Wu

doi:10.1155/2021/4852406

YQHX Alleviates H/R-Induced Cardiomyocyte Apoptosis by Downregulating miR-1

Evid Based Complement Alternat Med. 2021 Nov 2:2021:4852406. doi: 10.1155/2021/4852406. eCollection 2021.

Authors

Luandie Ge¹, Yaqi Fan¹, Lin Fu¹, Mengjiao Guo¹, Panxia Cao¹, Chaojie Peng¹, Linke Wu¹, Lihua Han², Hong Wu^{1

2

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Affiliations

¹ Graduate School, Henan University of Chinese Medicine, Zhengzhou 450046, China.
² Institute of Cardiovascular Disease, Henan University of Chinese Medicine, Zhengzhou 450002, China.
³ Laboratory of Cell Imaging, Henan University of Chinese Medicine, Zhengzhou 450002, China.

Abstract

Yiqi Huoxue granule (YQHX) inhibits cardiomyocyte apoptosis in myocardial ischemia-reperfusion injury (MIRI); however, the underlying mechanism is unknown. In this study, hypoxia-reoxygenation (H/R) models were established using rat myocardial primary cells and H9c2 cells, lactate dehydrogenase (LDH), and creatine kinase (CK) levels and cardiomyocyte apoptosis were determined. LDH release, CK activity, caspase-3 activation, mRNA and protein ratio of Bax/Bcl-2, and miR-1 expression were significantly higher (p < 0.01) in the H/R model of rat myocardial primary cells and H9c2 cells compared with the control group and was inhibited by YQHX treatment (p < 0.01 or p < 0.05). We also found that miR-1 overexpression could enhance apoptosis in cardiomyocytes, whereas apoptosis could be reduced by YQHX treatment (p < 0.01). In conclusion, YQHX alleviates H/R-induced cardiomyocyte apoptosis by inhibiting miR-1 expression, suggesting the potential of YQHX in preventing MIRI.