Sinomenine Inhibits the Progression of Bladder Cancer Cells by Downregulating LncRNA-HEIH Expression

Haili Xu; Jiayan Dong; Junhui Hou; Rui Gao

doi:10.1155/2021/4699529

Sinomenine Inhibits the Progression of Bladder Cancer Cells by Downregulating LncRNA-HEIH Expression

Evid Based Complement Alternat Med. 2021 Nov 1:2021:4699529. doi: 10.1155/2021/4699529. eCollection 2021.

Authors

Haili Xu¹, Jiayan Dong², Junhui Hou³, Rui Gao¹

Affiliations

¹ Department of Oncology, Jinan Municipal Hospital of Traditional Chinese Medicine, Jinan 250012, China.
² Department of Tumor Radiotherapy, Zhangqiu District People's Hospital, Jinan 250200, China.
³ Department of Tumor Radiotherapy (III), Affiliated Qingdao Center Hospital, Qingdao University, Qingdao 2266000, China.

Abstract

Background: Sinomenine has been reported to effectively repress the progression of lung cancer and breast cancer. However, the effects of sinomenine in bladder cancer are not well understood. The purpose of this study was to evaluate the effects of sinomenine in bladder cancer.

Methods: The mRNA expression of HEIH in bladder cancer cells was measured by RT-qPCR. T24 and SW780 cells were treated with sinomenine for 24 hours. Cell viability was detected by the MTT assay. Cell migration and invasion were detected by the transwell assay. Western blotting assay was performed to assess the protein expression of Bcl-2, Bax, and caspase-3.

Results: Sinomenine significantly suppressed cell viability in T24 and SW780 cells. Moreover, cell migration and invasion were significantly inhibited by sinomenine. Sinomenine accelerated the expression of Bax and caspase-3 but decreased the expression of Bcl-2. HEIH was upregulated in bladder cancer cells compared with normal bladder epithelial cells. Besides this, we noticed that HEIH knockdown blocked cell proliferation, migration, and invasion but facilitated cell apoptosis in bladder cancer cells. Additionally, HEIH reversed the suppression of the progression induced by sinomenine.

Conclusion: Sinomenine was observed to suppress cell progression of bladder cancer cells by inhibiting HEIH expression. Our findings suggested that the use of sinomenine might be an effective treatment for bladder cancer.