Evaluation and development of a novel pre-treatment method for mulberry leaves to enhance their bioactivity via enzymatic degradation of GAL-DNJ to DNJ

Soo Takasu; Isabella Supardi Parida; Yoshihiro Kojima; Toshiyuki Kimura; Kiyotaka Nakagawa

doi:10.1039/d1fo02307a

Evaluation and development of a novel pre-treatment method for mulberry leaves to enhance their bioactivity via enzymatic degradation of GAL-DNJ to DNJ

Food Funct. 2021 Dec 13;12(24):12250-12255. doi: 10.1039/d1fo02307a.

Authors

Soo Takasu^{1

2}, Isabella Supardi Parida¹, Yoshihiro Kojima³, Toshiyuki Kimura⁴, Kiyotaka Nakagawa¹

Affiliations

¹ Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, 980-8572, Japan. kiyotaka.nakagawa.c1@tohoku.ac.jp.
² Laboratory of Pharmaceutical Analytical Chemistry, Gifu Pharmaceutical University, Gifu, 501-1196, Japan.
³ Minato Pharmaceutical Co., Ltd, Tokyo 104-0061, Japan.
⁴ Advanced Analysis Center, National Agriculture and Food Research Organization, Ibaraki 305-8642, Japan.

PMID: 34755739
DOI: 10.1039/d1fo02307a

Abstract

Mulberry leaves are rich in 1-deoxynojirimycin (DNJ) and 2-O-α-D-galactopyranosyl-deoxynojirimycin (GAL-DNJ). Compared to DNJ, the bioactive potency of GAL-DNJ is low. We proposed that the conversion of GAL-DNJ into DNJ may improve its bioavailability. We evaluated this hypothesis and constructed a novel enzymatic-based method to induce the hydrolysis of GAL-DNJ to DNJ in order to improve the therapeutic potency of mulberry leaves.

MeSH terms

Administration, Oral
Animals
Antioxidants / administration & dosage
Antioxidants / chemistry
Antioxidants / pharmacology*
Biological Availability
Functional Food
Humans
Hydrolysis
Intestinal Absorption / drug effects
Morus*
Plant Leaves
Rats
Rats, Sprague-Dawley

Substances

Antioxidants