A simple protocol to produce mature human-induced pluripotent stem cell-derived cardiomyocytes

STAR Protoc. 2021 Oct 25;2(4):100912. doi: 10.1016/j.xpro.2021.100912. eCollection 2021 Dec 17.

Abstract

When cultured under typical conditions, human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are structurally and functionally immature. We have previously demonstrated that culture of hiPSC-CMs in maturation medium containing fatty acids, in combination with culture on micropatterned surfaces, produces cells that demonstrate a more mature phenotype compared to standard approaches. Here, we show in detail the steps needed to produce mature hiPSC-CMs. Compared with many approaches, our protocol is relatively simple and can be easily adapted to new laboratories. For complete details on the use and execution of this protocol, please refer to Knight et al. (2021).

Keywords: Cell Biology; Cell Differentiation; Cell culture; Stem Cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Culture Techniques / methods*
  • Cell Differentiation / physiology
  • Cells, Cultured
  • Culture Media / chemistry
  • Culture Media / metabolism
  • Fatty Acids / chemistry
  • Fatty Acids / metabolism
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Myocytes, Cardiac / cytology*

Substances

  • Culture Media
  • Fatty Acids