Starting a new recombinant protein production project in Escherichia coli

Dianela Aguilar Lucero; Alejo Cantoia; Eduardo A Ceccarelli; Germán L Rosano

doi:10.1016/bs.mie.2021.08.019

Starting a new recombinant protein production project in Escherichia coli

Methods Enzymol. 2021:659:3-18. doi: 10.1016/bs.mie.2021.08.019. Epub 2021 Sep 22.

Authors

Dianela Aguilar Lucero¹, Alejo Cantoia¹, Eduardo A Ceccarelli¹, Germán L Rosano²

Affiliations

¹ Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina.
² Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina. Electronic address: rosano@ibr-conicet.gov.ar.

PMID: 34752291
DOI: 10.1016/bs.mie.2021.08.019

Abstract

One of the goals in recombinant protein production in Escherichia coli is to maximize productivity. High volumetric and specific yields can be reached after careful selection of expression strains and optimization of cultivation parameters. In this chapter, we review the many tools available to make the most out of this versatile microbial cell factory. Useful guidelines and options for troubleshooting production are presented.

Keywords: Cultivation factors; Escherichia coli; Inducer; Protein aggregation; Recombinant proteins.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Escherichia coli* / genetics
Escherichia coli* / metabolism
Recombinant Proteins / metabolism

Substances

Recombinant Proteins