Sulfur is an element that is indispensable throughout the growth of plants. In plant cells, reactive sulfur species (RSS) play a vital role in maintaining cellular redox homeostasis and signal transduction. There is demand accordingly for a simple, highly selective, and sensitive method of RSS detection and imaging for monitoring dynamic changes and clarifying the biological functions of RSS in plant systems. Fluorescent analysis based on organic small-molecule fluorescent probes is an effective and specific approach to tracking plant RSS characteristics. This perspective summarizes the recent progress regarding organic small-molecule fluorescent probes for RSS monitoring, including small-molecule biological thiols, hydrogen sulfide, and sulfane sulfurs, in plants; it also discusses their response mechanism toward RSS and their imaging applications in plants across the agricultural chemistry field.
Keywords: agricultural chemistry; detection in plants; fluorescent probes; in vivo bioimaging; reactive sulfur species.