To determine the effects of epigallocatechin-3-gallate (EGCG) on the cryopreservation of bovine semen, epigallocatechin-3-gallate dissolved with double distilled water to 0.2, 0.4, 0.6 and 0.8 mg/ml were added to the cryopreservation diluent of the bull semen. Then, we used computer-assisted analysis of semen kinematic parameters, staining method to detect membrane function, acrosome integrity, enzyme-linked immunosorbent assay to detect catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehydes (MDA) and reactive oxygen levels. The results showed that adding 0.6 mg/L of epigallocatechin-3-gallate could improve the cryopreserved sperm quality, which significantly increased the total motility, distance average path, distance straight line, distance curved line, average path velocity, curvilinear-velocity, straight-line velocity, amplitude of lateral head displacement and beat/cross frequency, as well as sperm CAT, GSH-Px and SOD levels (p < 0.05), whilst reducing the reactive oxygen species and MDA levels (p < 0.05). Hence, these results indicate that the addition of 0.6 mg/ml of EGCG has a protective effect on the cryopreservation of the bovine semen.
Keywords: bull semen; cryopreservation; epigallocatechin-3-gallate; sperm analysis.
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