Cerium oxide nanoparticle conjugation to microRNA-146a mechanism of correction for impaired diabetic wound healing

Nanomedicine. 2022 Feb:40:102483. doi: 10.1016/j.nano.2021.102483. Epub 2021 Nov 6.

Abstract

Diabetic wounds represent a significant healthcare burden and are characterized by impaired wound healing due to increased oxidative stress and persistent inflammation. We have shown that CNP-miR146a synthesized by the conjugation of cerium oxide nanoparticles (CNP) to microRNA (miR)-146a improves diabetic wound healing. CNP are divalent metal oxides that act as free radical scavenger, while miR146a inhibits the pro-inflammatory NFκB pathway, so CNP-miR146a has a synergistic role in modulating both oxidative stress and inflammation. In this study, we define the mechanism(s) by which CNP-miR146a improves diabetic wound healing by examining immunohistochemical and gene expression analysis of markers of inflammation, oxidative stress, fibrosis, and angiogenesis. We have found that intradermal injection of CNP-miR146a increases wound collagen, enhances angiogenesis, and lowers inflammation and oxidative stress, ultimately promoting faster closure of diabetic wounds.

Keywords: Angiogenesis; CNP-miR146a; Cerium oxide nanoparticle (CNP); Diabetic wounds; Inflammation; Oxidative stress.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cerium* / chemistry
  • Cerium* / pharmacology
  • Diabetes Mellitus*
  • Humans
  • MicroRNAs* / metabolism
  • Nanoparticles* / chemistry
  • Wound Healing

Substances

  • MicroRNAs
  • Cerium
  • ceric oxide