Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes

STAR Protoc. 2021 Oct 8;2(4):100859. doi: 10.1016/j.xpro.2021.100859. eCollection 2021 Dec 17.

Abstract

Multiple strategies have been developed to efficiently differentiate human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Here, we describe a protocol for measuring three key functional parameters of hiPSC-CMs, including contractile function, calcium (Ca2+) handling, and action potential. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021).

Keywords: Cell Biology; Cell culture; Microscopy; Stem Cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Action Potentials / physiology*
  • Calcium / metabolism*
  • Cells, Cultured
  • Cytological Techniques / methods*
  • Humans
  • Induced Pluripotent Stem Cells* / cytology
  • Induced Pluripotent Stem Cells* / metabolism
  • Induced Pluripotent Stem Cells* / physiology
  • Myocytes, Cardiac* / cytology
  • Myocytes, Cardiac* / metabolism
  • Myocytes, Cardiac* / physiology

Substances

  • Calcium