Fabrication of Antimicrobial Multilayered Nanofibrous Scaffolds-Loaded Drug via Electrospinning for Biomedical Application

Qi Liu; Hengmin Jia; Wenchong Ouyang; Yan Mu; Zhengwei Wu

doi:10.3389/fbioe.2021.755777

Fabrication of Antimicrobial Multilayered Nanofibrous Scaffolds-Loaded Drug via Electrospinning for Biomedical Application

Front Bioeng Biotechnol. 2021 Oct 20:9:755777. doi: 10.3389/fbioe.2021.755777. eCollection 2021.

Authors

Qi Liu¹, Hengmin Jia², Wenchong Ouyang¹, Yan Mu², Zhengwei Wu^{1

3}

Affiliations

¹ School of Nuclear Science and Technology, University of Science and Technology of China, Hefei, China.
² Department of Infection Control and Prevention, The First Affiliated Hospital of University of Science and Technology of China, Hefei, China.
³ CAS Key Laboratory of Geospace Environment, University of Science and Technology of China, Hefei, China.

Abstract

Nanofibers prepared by biobased materials are widely used in the field of biomedicine, owing to outstanding biocompatibility, biodegradable characters, and excellent mechanical behavior. Herein, we fabricated multilayered nanofibrous scaffolds in order to improve the performance of drug delivery. The composite layer-by-layer scaffolds were incorporated by hydrophobic poly(l-lactic acid) (PLA): polycaprolactone (PCL) and hydrophilic poly(vinyl alcohol) (PVA) nanofibers via multilayer electrospinning. Morphological and structural characteristics of the developed scaffolds measured by scanning electron microscopy (SEM), and transmission electron microscopy (TEM) confirmed smooth and uniform fibers ranging in nanometer scale. The differences in contact angles and Fourier transform infrared spectrum (FTIR) between single-layered PVA nanofibers and multilayered scaffolds verified the existence of PLA: PCL surface. In vitro biodegradable and drug release analysis depicted multilayered scaffolds had good biodegradability and potential for medical application. Due to the model drug incorporation, scaffolds exhibited good antibacterial activity against Escherichia coli and Staphylococcus aureus by the zone of inhibition test. These results revealed that the multilayered scaffolds were proved to be desirable antibacterial materials for biomedical application.

Keywords: antimicrobial; biodegradable; drug delivery; multilayered nanofibers; poly(vinyl alcohol).