Photovoltaic optoelectronic tweezers are a useful platform with many applications in optical manipulation and nanotechnology. They are based on electrical forces associated with the bulk photovoltaic effect presented by certain ferroelectric crystals, such as Fe doped lithium niobate. This manipulation technique has experienced huge developments in recent years, although its use in biology and biomedicine is still scarce. Recently, a novel strategy has been reported that extends the platform capabilities to the manipulation of polar droplets, such as water and aqueous bio-droplets, promising great potential for biological applications. In this work, we are taking this challenge, addressing the manipulation of cells and macromolecules contained inside the droplets by optoelectronic ferroelectric platforms. On the one hand, experiments of photoelectric induced migration of DNA and sperm droplets have been successfully developed and the corresponding droplet dynamics have been analyzed in depth. From this analysis, parameters of the biomaterial such as its concentration and its electrical charge have been evaluated, showing the sensing capabilities of the platform. In fact, the charge of sperm cells has been demonstrated to be negative, and the relative sperm concentration of the samples determined. On the other hand, experiments on the light-induced merging of two droplets have been carried out. Specifically, sperm droplets are mixed with droplets containing acridine orange, a convenient dye for visualization purposes. The spermatozoa become clearly visible in the final droplet through fluorescence imaging. The results point out the multiple possibilities of application of the optoelectronic ferroelectric platform in biology and biomedicine including the development of "lab on a chip" devices. Hence, these capabilities introduce these platforms as an efficient tool in biotechnology.
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