Photodynamic Therapy of Novel Photosensitizer Ameliorates TNBS-Induced Ulcerative Colitis via Inhibition of AOC1

Yumei Rong; Ge Hong; Na Zhu; Yang Liu; Yong Jiang; Tianjun Liu

doi:10.3389/fphar.2021.746725

Photodynamic Therapy of Novel Photosensitizer Ameliorates TNBS-Induced Ulcerative Colitis via Inhibition of AOC₁

Front Pharmacol. 2021 Oct 21:12:746725. doi: 10.3389/fphar.2021.746725. eCollection 2021.

Authors

Yumei Rong¹, Ge Hong¹, Na Zhu¹, Yang Liu¹, Yong Jiang², Tianjun Liu¹

Affiliations

¹ Tianjin Key Laboratory of Biomedical Material, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China.
² Department of Gastroenterology, The Second Hospital of Tianjin Medical University, Tianjin, China.

Abstract

Ulcerative colitis (UC), a chronic, nonspecific inflammatory bowel disease characterized by continuous and diffuse inflammatory changes in the colonic mucosa, requires novel treatment method. Photodynamic therapy (PDT), as a promising physico-chemical treatment method, were used to treat UC rats' model with novel photosensitizer LD₄ in this paper, the treatment effect and mechanism was investigated. LD₄-PDT could improve the survival rate of 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced UC model rats, decrease expression of interleukin (IL)-6, IL-1, tumor necrosis factor (TNF)-α, malondialdehyde (MDA), myeloperoxidase (MPO) and increase the expression of glutathione (GSH) and superoxide oxidase (SOD), while protecting the integrity of the intestinal epithelium. LD₄-PDT treatment could rebuild the intestinal microflora composition and reprogram the colonic protein profiles in TNBS-induced rats to almost the normal state. Proteomics analysis based upon TNBS-induced UC model rats revealed that Amine oxidase copper-containing 1 (AOC₁) was a potential target of LD₄-PDT. Novel photosensitizer agent LD₄-PDT represents an efficient treatment method for UC, and AOC₁ may be a promising target.

Keywords: AOC1; gut microbiota; novel photosensitizer; photodynamic therapy; ulcerative colitis.