Microbial commensalism-assisted fast acclimation of HAP-anammox granules to dewatered liquid of dry methane fermentation

Ying Song; Zhen Ma; Runda Du; Yan Guo; Yu Qin; Jun Tanno; Wei-Kang Qi; Yu-You Li

doi:10.1016/j.biortech.2021.126238

Microbial commensalism-assisted fast acclimation of HAP-anammox granules to dewatered liquid of dry methane fermentation

Bioresour Technol. 2022 Jan;344(Pt B):126238. doi: 10.1016/j.biortech.2021.126238. Epub 2021 Oct 28.

Authors

Ying Song¹, Zhen Ma², Runda Du¹, Yan Guo¹, Yu Qin¹, Jun Tanno³, Wei-Kang Qi⁴, Yu-You Li⁵

Affiliations

¹ Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, 6-6-06 Aza, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan.
² Graduate School of Environmental Studies, Tohoku University, 6-6-06 Aramaki Aza Aoba, Aoba-ku, Sendai, Miyagi 980-8579, Japan.
³ Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, 6-6-06 Aza, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan; Department of Civil and Environmental Engineering, National Institute of Technology, Fukushima College, Fukushima 970-8034, Japan.
⁴ National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
⁵ Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, 6-6-06 Aza, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan; Graduate School of Environmental Studies, Tohoku University, 6-6-06 Aramaki Aza Aoba, Aoba-ku, Sendai, Miyagi 980-8579, Japan. Electronic address: gyokuyu.ri.a5@tohoku.ac.jp.

PMID: 34743991
DOI: 10.1016/j.biortech.2021.126238

Abstract

The treatment of a dewatered liquid of dry fermentation via the anammox process was investigated in the present study. Fast acclimation was established: within 2-months of operation, nitrogen removal rate reached 5 times (5.5 g-N/L/d) higher than it was at startup, which was achieved by inoculation with cold-stored HAP-anammox granules and inhibition control. The specific anammox activity of the dewatered liquid was highly improved and quite comparable to that of synthetic wastewater. Ca. Kuenenia with the relative abundance of 31.1% was revealed to be the only anammox genre and maintained its dominance throughout the operation. Simultaneously, Ca. D. denitrificans was proliferated, with its relative abundance increasing from 1.5% to 14.9%. The microbial co-occurrence network of HAP-anammox granules developed during the treatment of the dewatered liquid of dry fermentation. The experience of this work provides valuable strategies facilitating fast acclimation of the anammox process for the treatment of high-strength wastewater.

Keywords: Ca. D. denitrificans; Ca. Kuenenia; Fermentation liquid; HAP-anammox granules.

MeSH terms

Acclimatization
Ammonium Compounds*
Anaerobic Ammonia Oxidation
Bioreactors
Denitrification*
Fermentation
Methane
Nitrogen
Oxidation-Reduction
Sewage
Symbiosis
Wastewater

Substances

Ammonium Compounds
Sewage
Waste Water
Nitrogen
Methane