CRISPR/Cas9 in lepidopteran insects: Progress, application and prospects

Jiang-Jie Li; Yan Shi; Ji-Nan Wu; Hao Li; Guy Smagghe; Tong-Xian Liu

doi:10.1016/j.jinsphys.2021.104325

CRISPR/Cas9 in lepidopteran insects: Progress, application and prospects

J Insect Physiol. 2021 Nov-Dec:135:104325. doi: 10.1016/j.jinsphys.2021.104325. Epub 2021 Oct 30.

Authors

Jiang-Jie Li¹, Yan Shi², Ji-Nan Wu³, Hao Li³, Guy Smagghe⁴, Tong-Xian Liu⁵

Affiliations

¹ Key Lab of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, Shandong 266109, PR China; Laboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium.
² Key Lab of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, Shandong 266109, PR China; Laboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium. Electronic address: shiyanyuanyi@aliyun.com.
³ Key Lab of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, Shandong 266109, PR China.
⁴ Laboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium. Electronic address: guy.smagge@ugent.be.
⁵ Key Lab of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, Shandong 266109, PR China. Electronic address: txliu@qau.edu.cn.

PMID: 34743972
DOI: 10.1016/j.jinsphys.2021.104325

Abstract

Clustered regularly spaced short palindrome repeats (CRISPR) structure family forms the acquired immune system in bacteria and archaea. Recent advances in CRISPR/Cas genome editing as derived from prokaryotes, confirmed the characteristics of robustness, high target specificity and programmability, and also revolutionized the insect sciences field. The successful application of CRISPR in a wide variety of lepidopteran insects, with a high genetic diversity, provided opportunities to explore gene functions, insect modification and pest control. In this review, we present a detailed overview on the recent progress of CRISPR in lepidopteran insects, and described the basic principles of the system and its application. Major interest is on wing development, pigmentation, mating, reproduction, sex determination, metamorphosis, resistance and silkworm breeding innovation. Finally, we outlined the limitations of CRISPR/Cas system and discussed its application prospects in lepidopteran insects.

Keywords: CRISPR/Cas9 system; Lepidoptera; Prospects; Research progress.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Bombyx / genetics
CRISPR-Cas Systems*
Gene Editing*
Lepidoptera / genetics*