Background: Biopolymers are promising candidates that can be fabricated into hydrophilic matrices and used for many applications due to their distinctive properties such as non-toxic, biodegradable, biocompatibility, and low cost. A promising composite of chitosan and carrageenan with self-crosslinking has been prepared.
Methods and results: In this study, a rigorous approach for an inexpensive and non-toxic combination of different amounts of clove oil with two polyelectrolytes including chitosan and carrageenan in the form of beads has been prepared. The structure and the surface morphology of the beads were investigated using FTIR, X-ray diffraction (XRD), and SEM. Moreover, antimicrobial, antiviral activity, and molecular docking were evaluated. Antibacterial results revealed that chitosan/carrageenan@clove oil beads have antimicrobial activity as well as chitosan/carrageenan without clove oil against Escherichia coli AATCC25922, Pseudomonas aeruginosa AATCC27853, Staphylococcus aureus ATCC25923, Bacillus subtilis AATCC6051, and Candida albicans ATCC90028. Furthermore, maximum non-toxic concentration (MNTC) of chitosan/carrageenan@clove oil beads was (31.25 μg ml-1 ) which exhibited promising antiviral activity against Herpes simplex virus-1 (HSV-1), and was significantly higher than chitosan/carrageenan without clove oil, where antiviral activity was 82.94% and 57.64%, respectively. Eventually, docking study and computational calculation have been used to show the reactivity of the molecules.
Conclusions: The developed chitosan/carrageenan@clove oil beads have shown promising properties to be used as carriers of drug delivery, tissue engineering, and regenerative medicine.
Keywords: antimicrobial; antiviral; beads; carrageenan; chitosan; clove oil; cytotoxicity.
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