Apart from oocyte quality, the media used has a significant effect on the production and quality of blastocysts produced in vitro. This study was designed to evaluate the replacement of serum with human amniotic membrane stem cells' conditioned medium (hAMSCs-CM) during bovine embryo culture on the quantity and quality of produced blastocysts. The in-vitro-produced embryos on the third day of IVC were randomly divided into the following culture groups: SOFaa + 5% FBS (Control), SOFaa + 5% hAMSCs-CM (5% CM), SOFaa + 2.5% hAMSCs-CM + 2.5% FBS (2.5% CM) and SOFaa + hAMSC co-culture (co-culture). The blastocyst and hatching rates, blastocyst cells number (the number of trophectoderm, inner cell mass and total cells), and the expression of some developmentally important genes (OCT4, PLAC8 and COX2 genes) in the treated groups, especially in the 5% CM, compared to the control had improved (p < .05). No significant difference was observed between groups for viability and hatching rate in vitrified-warmed blastocysts. Due to the positive effect of hAMSCs' conditioned medium (hAMSCs-CM) on blastocyst production, as well as its ease of preparation and the need to avoid the transmission of microbial contamination to the culture medium, hAMSCs-CM can be used as a suitable alternative to FBS during 3 to 8 days of bovine embryo culture.
Keywords: blastocyst; conditioned media; human amniotic membrane; stem cell.
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