Effect of Inonotus obliquus polysaccharide on composition of the intestinal flora in mice with acute endometritis

PLoS One. 2021 Nov 5;16(11):e0259570. doi: 10.1371/journal.pone.0259570. eCollection 2021.

Abstract

Inonotus obliquus Polysaccharide (IOP) is a large molecule extracted from Inonotus obliqus, a medicinal fungus, which has a wide range of biological activities and has been shown to be associated with inflammation. The purpose of this study is to investigate whether IOP can help to reduce acute endometritis by regulating intestinal flora. We observed pathological changes in mice with endometritis following treatment with IOP and evaluated changes in the levels of interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α), and further studied the effects of IOP on the intestinal flora of endometritis mice using 16S rRNA high-throughput sequencing. The results showed that IOP improved the condition of uterine tissues and reduced the release of pro-inflammatory cytokines. Meanwhile, the 16S rRNA sequencing results showed that IOP could regulate the changes in intestinal microflora at the level of genera, possibly by changing the relative abundance of some genera.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Animals
  • Endometritis*
  • Female
  • Gastrointestinal Microbiome*
  • Interleukin-6
  • Mice
  • RNA, Ribosomal, 16S
  • Uterus

Substances

  • Interleukin-6
  • RNA, Ribosomal, 16S

Grants and funding

This work was supported by the Chengdu Normal University scientific research project [No.111-153701] (Acceptor: Binhong Hu) and Chengdu Normal University agricultural ecology and green food development project [CSCXTD2020B11] (Acceptor: Songqing Liu). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.