Cross-platform transcriptomic profiling of the response to recombinant human erythropoietin

Sci Rep. 2021 Nov 4;11(1):21705. doi: 10.1038/s41598-021-00608-9.

Abstract

RNA-seq has matured and become an important tool for studying RNA biology. Here we compared two RNA-seq (MGI DNBSEQ and Illumina NextSeq 500) and two microarray platforms (GeneChip Human Transcriptome Array 2.0 and Illumina Expression BeadChip) in healthy individuals administered recombinant human erythropoietin for transcriptome-wide quantification of differential gene expression. The results show that total RNA DNB-seq generated a multitude of target genes compared to other platforms. Pathway enrichment analyses revealed genes correlate to not only erythropoiesis and oxygen transport but also a wide range of other functions, such as tissue protection and immune regulation. This study provides a knowledge base of genes relevant to EPO biology through cross-platform comparisons and validation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Erythropoiesis / genetics
  • Erythropoietin / genetics
  • Gene Expression / genetics
  • Gene Expression Profiling / methods*
  • High-Throughput Nucleotide Sequencing / methods
  • Humans
  • Oligonucleotide Array Sequence Analysis / methods*
  • RNA / genetics
  • RNA-Seq / methods
  • Sequence Analysis, RNA / methods*
  • Transcriptome / genetics

Substances

  • Erythropoietin
  • RNA