Sensitivity of photoelctrocehmical aptasensor using spiral nanorods for detecting antiobiotic levels in experimental and real samples

Yimeng Sun; Cong Ma; Man Wu; Chunping Jia; Shilun Feng; Jianlong Zhao; Lijuan Liang

doi:10.1016/j.talanta.2021.122930

Sensitivity of photoelctrocehmical aptasensor using spiral nanorods for detecting antiobiotic levels in experimental and real samples

Talanta. 2022 Jan 15:237:122930. doi: 10.1016/j.talanta.2021.122930. Epub 2021 Oct 4.

Authors

Yimeng Sun¹, Cong Ma², Man Wu¹, Chunping Jia¹, Shilun Feng³, Jianlong Zhao¹, Lijuan Liang⁴

Affiliations

¹ State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China.
² State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China; School of Information Science and Technology, ShanghaiTech University, Shanghai, 201210, China.
³ State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China. Electronic address: shilun.feng@mail.sim.ac.cn.
⁴ State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China. Electronic address: llj@mail.sim.ac.cn.

PMID: 34736667
DOI: 10.1016/j.talanta.2021.122930

Abstract

Given increasing concern regarding antibiotic environmental contamination, there is immediate need to monitor antibiotic levels to effectively control pollution. In this study, we used a photoelectrochemical aptasensor based on TiO₂@MoS₂ spiral nanoarrays to detect chloramphenicol (CAP) in antibiotics. Nanoarrays were directly grown on fluorine-doped tin oxide (FTO) conductive glass with excellent biochemical stability, while aptamer-SH were immobilized by chemical binding on a synthetic TiO₂@MoS₂ nanoarray. Results show that the photocurrents were reduced in the presence of photoelectrochemistry associated with specific selection of aptamer for CAP. When the measurement of the fabricated nanomaterial chip was carried out using a three-electrode system, we found a highly specific and stable detection of chloramphenicol that ranged between 0.1 pM and 1 μM, with the detection limit of 0.1 pM. In addition, we obtained satisfactory results when real sample were used to validate the potential of photoelectrochemical (PEC) aptasensor for detecting chloramphenicol content in milk. Our results demonstrate that photoelectrochemical aptasensor is conducive to the development of less toxic multifunctional nanomaterials, making the biosensor more robust and environmentally friendly. Therefore, photoelectrochemical aptasensor can be widely applied in the field of environmental monitoring.

Keywords: Antibiotics detection; Aptasensor; Chloramphenicol; Photoelectrochemistry(PEC).

MeSH terms

Aptamers, Nucleotide*
Biosensing Techniques*
Electrochemical Techniques
Limit of Detection
Nanotubes*
Titanium

Substances

Aptamers, Nucleotide
Titanium