Optimisation of methodology for whole genome sequencing of Measles Virus directly from patient specimens

Helene Schulz; Joanne Hiebert; Jasmine Frost; Elizabeth McLachlan; Alberto Severini

doi:10.1016/j.jviromet.2021.114348

Optimisation of methodology for whole genome sequencing of Measles Virus directly from patient specimens

J Virol Methods. 2022 Jan:299:114348. doi: 10.1016/j.jviromet.2021.114348. Epub 2021 Oct 30.

Authors

Helene Schulz¹, Joanne Hiebert¹, Jasmine Frost¹, Elizabeth McLachlan¹, Alberto Severini²

Affiliations

¹ Department of Medical Microbiology and Infectious Diseases, Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, Canada.
² Department of Medical Microbiology and Infectious Diseases, Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, Canada; National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada. Electronic address: alberto.severini@canada.ca.

PMID: 34728271
DOI: 10.1016/j.jviromet.2021.114348

Abstract

In an era of decreasing genetic diversity of Measles Virus (MeV), effective surveillance requires a higher-resolution genotyping method or whole genome sequencing (WGS) to document elimination. Through optimization of MeV WGS protocol, we developed a MeV-specific probe enrichment method that allows next generation sequencing from clinical specimens. With the probe enrichment method, 70% of specimens can be sequenced at a read depth of greater than 10 reads with minimal off-target sequences.

Keywords: Measles Virus; Next generation sequencing; complete genome sequence; probe enrichment.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
High-Throughput Nucleotide Sequencing*
Humans
Measles virus* / genetics
Whole Genome Sequencing / methods