Protocol for measuring NLRC4 inflammasome activation and pyroptosis in murine bone-marrow-derived macrophages

STAR Protoc. 2021 Oct 19;2(4):100894. doi: 10.1016/j.xpro.2021.100894. eCollection 2021 Dec 17.

Abstract

NLR family CARD domain containing protein 4 (NLRC4) inflammasome activation and the associated pyroptosis are critical for protection against infection by bacterial pathogens. This protocol presents a detailed procedure to activate and measure NLRC4 inflammasome activation and pyroptosis upon Salmonella Typhimurium infection. The techniques can be adapted to monitoring the activation of other types of inflammasomes and pathogenic stimuli. For comprehensive details on the use and execution of this protocol, please refer to Dong et al. (2021).

Keywords: Cell Biology; Immunology; Microbiology; Microscopy; Molecular Biology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis Regulatory Proteins* / analysis
  • Apoptosis Regulatory Proteins* / metabolism
  • Calcium-Binding Proteins* / analysis
  • Calcium-Binding Proteins* / metabolism
  • Inflammasomes* / analysis
  • Inflammasomes* / metabolism
  • Macrophages* / cytology
  • Macrophages* / metabolism
  • Mice
  • Microscopy, Fluorescence
  • Pyroptosis / physiology*

Substances

  • Apoptosis Regulatory Proteins
  • Calcium-Binding Proteins
  • Inflammasomes
  • Ipaf protein, mouse