Metabolic Profile in Neonatal Pig Hearts

Pengsheng Li; Fan Li; Ling Tang; Wenjing Zhang; Yan Jin; Haiwei Gu; Wuqiang Zhu

doi:10.3389/fcvm.2021.763984

Metabolic Profile in Neonatal Pig Hearts

Front Cardiovasc Med. 2021 Oct 14:8:763984. doi: 10.3389/fcvm.2021.763984. eCollection 2021.

Authors

Pengsheng Li¹, Fan Li^{1

2}, Ling Tang¹, Wenjing Zhang^{3

4

5}, Yan Jin³, Haiwei Gu^{3

4

5}, Wuqiang Zhu¹

Affiliations

¹ Department of Cardiovascular Diseases, Physiology and Biomedical Engineering, Center for Regenerative Medicine, Mayo Clinic Arizona, Scottsdale, AZ, United States.
² Laboratory of Regenerative Medicine in Sports Science, School of Physical Education and Sports Science, South China Normal University, Guangzhou, China.
³ College of Health Solutions, Arizona State University, Phoenix, AZ, United States.
⁴ Department of Environmental Health Sciences, Robert Stempel College of Public Health and Social Work, Florida International University, Miami, FL, United States.
⁵ Department of Cellular Biology and Pharmacology, Herbert Wertheim College of Medicine, Center for Translational Science, Florida International University, Port St. Lucie, FL, United States.

Abstract

We evaluated the metabolic profile in pig hearts at postnatal day 1, 3, 7, and 28 (P1, P3, P7, and P28, respectively) using a targeted liquid chromatography tandem mass spectrometry assay. Our data showed that there is a clear separation of the detected metabolites in P1 vs. P28 hearts. Active anabolisms of nucleotide and proteins were observed in P1 hearts when cardiomyocytes retain high cell cycle activity. However, the active posttranslational protein modification, metabolic switch from glucose to fatty acids, and the reduced ratio of collagen to total protein were observed in P28 hearts when cardiomyocytes withdraw from cell cycle.

Keywords: heart; metabolism; metabolomics; neonatal; pig.

Abstract

Grants and funding