An Explanation Based on Energy-Related Changes for Blue Mussel Mytilus edulis Coping With Seawater Acidification

Ying Guo; Bin Zhou; Tianli Sun; Yaya Zhang; Yongshun Jiang; You Wang

doi:10.3389/fphys.2021.761117

An Explanation Based on Energy-Related Changes for Blue Mussel Mytilus edulis Coping With Seawater Acidification

Front Physiol. 2021 Oct 14:12:761117. doi: 10.3389/fphys.2021.761117. eCollection 2021.

Authors

Ying Guo^{1

2}, Bin Zhou^{1

2}, Tianli Sun³, Yaya Zhang^{1

2}, Yongshun Jiang⁴, You Wang^{1

2}

Affiliations

¹ College of Marine Life Sciences, Ocean University of China, Qingdao, China.
² Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology, Qingdao, China.
³ National Marine Hazard Mitigation Service, Beijing, China.
⁴ Marine Science and Engineering College, Qingdao Agricultural University, Qingdao, China.

Abstract

As ocean acidification (OA) is gradually increasing, concerns regarding its ecological impacts on marine organisms are growing. Our previous studies have shown that seawater acidification exerted adverse effects on physiological processes of the blue mussel Mytilus edulis, and the aim of the present study was to obtain energy-related evidence to verify and explain our previous findings. Thus, the same acidification system (pH: 7.7 or 7.1; acidification method: HCl addition or CO₂ enrichment; experimental period: 21d) was set up, and the energy-related changes were assessed. The results showed that the energy charge (EC) and the gene expressions of cytochrome C oxidase (COX) reflecting the ATP synthesis rate increased significantly after acidification treatments. What's more, the mussels exposed to acidification allocated more energy to gills and hemocytes. However, the total adenylate pool (TAP) and the final adenosine triphosphate (ATP) in M. edulis decreased significantly, especially in CO₂ treatment group at pH 7.1. It was interesting to note that, TAP, ATP, and COXs gene expressions in CO₂ treatment groups were all significantly lower than that in HCl treatment groups at the same pH, verifying that CO₂-induced acidification exhibited more deleterious impacts on M. edulis, and ions besides H⁺ produced by CO₂ dissolution were possible causes. In conclusion, energy-related changes in M. edulis responded actively to seawater acidification and varied with different acidification conditions, while the constraints they had at higher acidification levels suggest that M. edulis will have a limited tolerance to increasing OA in the future.

Keywords: Mytilus edulis; adenosine triphosphate; cytochrome C oxidase; energy allocation; energy charge; seawater acidification; total adenylate pool.