Morphology, ontogeny and molecular phylogeny of a new urostylid ciliate, Bakuella (Pseudobakuella) guangdongica n. sp. (Ciliophora, Hypotrichia) from southern China

Jingbao Li; Yurui Wang; Hui Zhang; Saleh A Al-Farraj; Chen Shao; Jingyi Wang

doi:10.1016/j.ejop.2021.125795

Morphology, ontogeny and molecular phylogeny of a new urostylid ciliate, Bakuella (Pseudobakuella) guangdongica n. sp. (Ciliophora, Hypotrichia) from southern China

Eur J Protistol. 2021 Oct:81:125795. doi: 10.1016/j.ejop.2021.125795. Epub 2021 Apr 21.

Authors

Jingbao Li¹, Yurui Wang², Hui Zhang³, Saleh A Al-Farraj⁴, Chen Shao⁵, Jingyi Wang⁶

Affiliations

¹ Laboratory of Protozoological Biodiversity and Evolution in Wetland, College of Life Sciences, Shaanxi Normal University, Xi'an 710119, China; Key Laboratory for Space Bioscience and Biotechnology, Institute of Special Environmental Biophysics, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, China.
² Laboratory of Protozoological Biodiversity and Evolution in Wetland, College of Life Sciences, Shaanxi Normal University, Xi'an 710119, China.
³ Key Laboratory for Space Bioscience and Biotechnology, Institute of Special Environmental Biophysics, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, China.
⁴ Zoology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
⁵ Laboratory of Protozoological Biodiversity and Evolution in Wetland, College of Life Sciences, Shaanxi Normal University, Xi'an 710119, China. Electronic address: shaochen@snnu.edu.cn.
⁶ Laboratory of Protozoological Biodiversity and Evolution in Wetland, College of Life Sciences, Shaanxi Normal University, Xi'an 710119, China. Electronic address: Wangjingyi629@163.com.

PMID: 34717076
DOI: 10.1016/j.ejop.2021.125795

Abstract

The morphology, morphogenesis, and molecular phylogeny of Bakuella (Pseudobakuella) guangdongica n. sp., isolated from southern China, were investigated. The new species is characterized by a body length of 150-225 μm in vivo; 35-42 adoral membranelles; 3-5 buccal, two frontoterminal, 7-12 transverse and two pretransverse ventral cirri; midventral complex comprised of 10-20 pairs and two rows extending to transverse cirri; posterior part of marginal rows slightly overlapping; colorless cortical granules about 1 μm across, arranged in small groups; soil habitat. Its main ontogenetic features are: (1) in the proter, the parental adoral zone of membranelles is completely renewed by new structures; (2) in the opisthe, the oral primordium originates apokinetally, some old midventral cirri join the formation of frontoventral-transverse cirral anlagen; (3) the anlagen for marginal rows and dorsal kineties develop intrakinetally; and (4) the numerous macronuclear nodules fuse into a single mass before dividing. Phylogenetic analyses based on the SSU rDNA sequence suggest the non-monophyly of the genus Bakuella.

Keywords: Morphogenesis; Morphology; New ciliate; Phylogeny; Soil.

MeSH terms

China
Ciliophora* / genetics
DNA, Ribosomal
Hypotrichida* / genetics
Phylogeny

Substances

DNA, Ribosomal