Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells

Tereza Souralova; Zuzana Holubcova; Drahomira Kyjovska; Ales Hampl; Irena Koutna

doi:10.1016/j.scr.2021.102574

Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells

Stem Cell Res. 2021 Dec:57:102574. doi: 10.1016/j.scr.2021.102574. Epub 2021 Oct 15.

Authors

Tereza Souralova¹, Zuzana Holubcova², Drahomira Kyjovska³, Ales Hampl⁴, Irena Koutna⁵

Affiliations

¹ Masaryk University, Faculty of Medicine, Department of Histology and Embryology, Brno, Czech Republic; St. Anne's University Hospital, International Clinical Research Center, Cell and Tissue Engineering Facility, Brno, Czech Republic.
² Masaryk University, Faculty of Medicine, Department of Histology and Embryology, Brno, Czech Republic; Reprofit International, Clinic of Reproductive Medicine, Brno, Czech Republic.
³ Reprofit International, Clinic of Reproductive Medicine, Brno, Czech Republic.
⁴ Masaryk University, Faculty of Medicine, Department of Histology and Embryology, Brno, Czech Republic; St. Anne's University Hospital, International Clinical Research Center, Cell and Tissue Regeneration, Brno, Czech Republic.
⁵ Masaryk University, Faculty of Medicine, Department of Histology and Embryology, Brno, Czech Republic; St. Anne's University Hospital, International Clinical Research Center, Cell and Tissue Engineering Facility, Brno, Czech Republic. Electronic address: irena.koutna@med.muni.cz.

PMID: 34715503
DOI: 10.1016/j.scr.2021.102574

Abstract

Human embryonic stem cells (hESCs) represent a virtually unlimited source of cells suitable for a variety of biomedical applications. However, a diminishing allogeneic background and undefined culture conditions are essential for developing robust and replicable protocols for differentiation experiments, disease modeling, and drug testing. Therefore, here we report the generation of the two sex-discordant sibling hESC lines, MUNIe008-A and MUNIe009-A, using the mechanical biopsy of vitrified-thawed embryos under xeno- and feeder-free conditions. The presented approach is applicable for deriving high-quality clinical-grade hESC lines for cell replacement therapies.