Engineered bacteria for valorizing lignocellulosic biomass into bioethanol

Hamed Kazemi Shariat Panahi; Mona Dehhaghi; Somayeh Dehhaghi; Gilles J Guillemin; Su Shiung Lam; Mortaza Aghbashlo; Meisam Tabatabaei

doi:10.1016/j.biortech.2021.126212

Engineered bacteria for valorizing lignocellulosic biomass into bioethanol

Bioresour Technol. 2022 Jan;344(Pt A):126212. doi: 10.1016/j.biortech.2021.126212. Epub 2021 Oct 27.

Authors

Hamed Kazemi Shariat Panahi¹, Mona Dehhaghi², Somayeh Dehhaghi³, Gilles J Guillemin⁴, Su Shiung Lam⁵, Mortaza Aghbashlo⁶, Meisam Tabatabaei⁷

Affiliations

¹ Henan Province Engineering Research Center for Forest Biomass Value-added Products, School of Forestry, Henan Agricultural University, Zhengzhou, Henan, 450002, China; Neuroinflammation Group, Department of Biomedical Sciences, Faculty of Medicine, Health and Human Sciences, Macquarie University, NSW, Australia; Biofuel Research Team (BRTeam), Terengganu, Malaysia.
² Neuroinflammation Group, Department of Biomedical Sciences, Faculty of Medicine, Health and Human Sciences, Macquarie University, NSW, Australia; Biofuel Research Team (BRTeam), Terengganu, Malaysia; PANDIS.org, Australia.
³ Department of Agricultural Extension and Education, Tarbiat Modares University, Tehran 14115-336, Iran.
⁴ Neuroinflammation Group, Department of Biomedical Sciences, Faculty of Medicine, Health and Human Sciences, Macquarie University, NSW, Australia; PANDIS.org, Australia.
⁵ Henan Province Engineering Research Center for Forest Biomass Value-added Products, School of Forestry, Henan Agricultural University, Zhengzhou, Henan, 450002, China; Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries (AKUATROP), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia. Electronic address: lam@umt.edu.my.
⁶ Department of Mechanical Engineering of Agricultural Machinery, Faculty of Agricultural Engineering and Technology, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
⁷ Henan Province Engineering Research Center for Forest Biomass Value-added Products, School of Forestry, Henan Agricultural University, Zhengzhou, Henan, 450002, China; Biofuel Research Team (BRTeam), Terengganu, Malaysia; Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries (AKUATROP), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia; Microbial Biotechnology Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

PMID: 34715341
DOI: 10.1016/j.biortech.2021.126212

Abstract

Appropriate bioprocessing of lignocellulosic materials into ethanol could address the world's insatiable appetite for energy while mitigating greenhouse gases. Bioethanol is an ideal gasoline extender and is widely used in many countries in blended form with gasoline at specific ratios to improve fuel characteristics and engine performance. Although the bioethanol production industry has long been operational, finding a suitable microbial agent for the efficient conversion of lignocelluloses is still an active field of study. Among available microbial candidates, engineered bacteria may be promising ethanol producers while may show other desired traits such as thermophilic nature and high ethanol tolerance. This review provides the current knowledge on the introduction, overexpression, and deletion of the genes that have been performed in bacterial hosts to achieve higher ethanol yield, production rate and titer, and tolerance. The constraints and possible solutions and economic feasibility of the processes utilizing such engineered strains are also discussed.

Keywords: Bioethanol; Consolidated bioprocessing; Gasoline extender; Metabolic engineering; Second generation feedstock; Thermophilic bacteria.

Publication types

Review

MeSH terms

Bacteria* / genetics
Bacteria* / metabolism
Biomass
Fermentation
Lignin* / metabolism

Substances

lignocellulose
Lignin