Effects of nitrogen on the longitudinal and vertical patterns of the composition and potential function of bacterial and archaeal communities in the tidal mudflats

Abstract

Increasing attention has been focused on the diminishing health of coastal ecosystems. Understanding the effects of eutrophication on tidal flat ecosystems is beneficial for the restoration and management of coastal ecosystems. However, previous studies did not consider the effects of nitrogen on the structure and function of bacterial and archaeal communities in longitudinal and vertical profiles. Here, the diversity, composition, assembly mechanism, and potential metabolic function of the bacterial and archaeal communities were studied in two longitudinal tidal sections at different eutrophic levels. Nitrogen and salinity were the critical factors that influenced the bacterial and archaeal community composition using canonical correspondence and multivariate regression tree analyses. For the bacterial community, the higher nitrogen loading in tidal mudflats resulted in the convergence of diversity and structure in the longitudinal profile of bacteria, but divergence was detected in the vertical profile. For archaea, the diversity tended to be convergent in longitudinal and vertical profiles in the higher nitrogen area, but the change of structure was similar to that of bacteria. Besides the homogeneous processes influenced by salinity, the assembly process of the bacterial community was mainly influenced by heterogeneous selection (34.8%) and that of archaea by dispersal limitation (19.5%). However, the bacterial and archaeal communities in the higher nitrogen section presented more of an influence of heterogeneous selection (respectively, 39 and 5.6%) than that of the lower nitrogen section (respectively, 10 and 0.2%). Structural equation modeling indicated that nitrogen may have inhibited the effects of the bacterial community on nitrogen turnover in nitrogen-rich anoxic sediment environments, but may have strengthened the effect of the archaeal community on carbon metabolism compared to bacteria. This work deepens our understanding of the responses of bacterial and archaeal community structure and potential function to nitrogen pollution in tidal mudflats.

Keywords: Carbon metabolism; Eutrophication; Heterogeneity; Homogenization; Microbial community; Nitrogen fixation.