Fertilizer stabilizers reduce nitrous oxide emissions from agricultural soil by targeting microbial nitrogen transformations

Abstract

Nitrous oxide (N₂O) is a pollutant released from agriculture soils following N fertilizer application. N stabilizers, such as N-(n-butyl) thiophosphoric triamide (NBPT) and 3,4-dimethylpyrazole phosphate (DMPP) could mitigate these N₂O emissions when applied with fertilizer. Here, field experiments were conducted to investigate the microbial mechanisms by which NBPT and DMPP mitigate N₂O emissions following urea application. We determined dynamic N₂O emissions and inorganic N concentrations for two wheat seasons and combined this with metagenomic sequencing. Application of NBPT, DMPP, and both NBPT and DMPP together with urea decreased mean N₂O accumulative emissions by 77.8, 91.4 and 90.7%, respectively, compared with urea application alone, mainly via repressing the increase in NO₂^- concentration after N fertilization. Sequencing results indicated that urea application enriched microorganisms that were positively correlated with N₂O production, whereas N stabilizers enriched microorganisms that were negatively correlated with N₂O production. Furthermore, compared to urea application alone, NBPT with urea reduced the abundances of genes related to denitrification, including napA/nasA, nirS/nirK, and norBC, resulting in a higher soil NO₃^- pool. Conversely, DMPP application, either alone or together with NBPT, decreased the abundance of genes involved in ammonia oxidation and denitrification, including amoCAB, hao, napA/nasA, nirS/nirK, and norBC, and maintained a greater soil NH₄⁺ pool. Both N stabilizers resulted in similar abundances of nirABD-which is related to NO₂^- reducers-as when no N fertilizer was applied, which could prevent NO₂^- accumulation, consequently mitigating N₂O emissions. These findings suggest that the high effectiveness of N stabilizers on mitigating N₂O emissions could be attributed to changes to soil microbial communities and N-cycling functional genes to control the by-product or intermediate products of microbial N-cycling processes in agricultural soils.

Keywords: 3,4-dimethylpyrazole phosphate (DMPP); Functional genes; N cycling; N-(n-butyl) thiophosphoric triamide (NBPT); Shotgun metagenomics; Soil microbial communities.