Development of a Transformable Fast-Flowering Mini-Maize as a Tool for Maize Gene Editing

Morgan E McCaw; Keunsub Lee; Minjeong Kang; Jacob D Zobrist; Mercy K Azanu; James A Birchler; Kan Wang

doi:10.3389/fgeed.2020.622227

Development of a Transformable Fast-Flowering Mini-Maize as a Tool for Maize Gene Editing

Front Genome Ed. 2021 Jan 11:2:622227. doi: 10.3389/fgeed.2020.622227. eCollection 2020.

Authors

Morgan E McCaw^{1

2}, Keunsub Lee^{1

2}, Minjeong Kang^{1

2

3}, Jacob D Zobrist^{1

2

4}, Mercy K Azanu^{1

2

3}, James A Birchler^{2

5}, Kan Wang^{1

2}

Affiliations

¹ Department of Agronomy, Iowa State University, Ames, IA, United States.
² Crop Bioengineering Center, Iowa State University, Ames, IA, United States.
³ Interdepartmental Plant Biology Major, Iowa State University, Ames, IA, United States.
⁴ Interdepartmental Genetics and Genomics Major, Iowa State University, Ames, IA, United States.
⁵ Division of Biological Sciences, University of Missouri, Columbia, MO, United States.

Abstract

Maize (Zea mays ssp. mays) is a popular genetic model due to its ease of crossing, well-established toolkits, and its status as a major global food crop. Recent technology developments for precise manipulation of the genome are further impacting both basic biological research and biotechnological application in agriculture. Crop gene editing often requires a process of genetic transformation in which the editing reagents are introduced into plant cells. In maize, this procedure is well-established for a limited number of public lines that are amenable for genetic transformation. Fast-Flowering Mini-Maize (FFMM) lines A and B were recently developed as an open-source tool for maize research by reducing the space requirements and the generation time. Neither line of FFMM were competent for genetic transformation using traditional protocols, a necessity to its status as a complete toolkit for public maize genetic research. Here we report the development of new lines of FFMM that have been bred for amenability to genetic transformation. By hybridizing a transformable maize genotype high Type-II callus parent A (Hi-II A) with line A of FFMM, we introgressed the ability to form embryogenic callus from Hi-II A into the FFMM-A genetic background. Through multiple generations of iterative self-hybridization or doubled-haploid method, we established maize lines that have a strong ability to produce embryogenic callus from immature embryos and maintain resemblance to FFMM-A in flowering time and stature. Using an Agrobacterium-mediated standard transformation method, we successfully introduced the CRISPR-Cas9 reagents into immature embryos and generated transgenic and mutant lines displaying the expected mutant phenotypes and genotypes. The transformation frequencies of the tested genotypes, defined as the numbers of transgenic event producing T1 seeds per 100 infected embryos, ranged from 0 to 17.1%. Approximately 80% of transgenic plants analyzed in this study showed various mutation patterns at the target site. The transformable FFMM line, FFMM-AT, can serve as a useful genetic and genomic resource for the maize community.

Keywords: Agrobacterium-mediated transformation; CRISPR; Zea mays; embryogenic callus; gene editing; transgenesis.