Therapeutic Effects of Ethanolic Extract of Polygonum limbatum meism Against Reproductive Toxicity Induced by Cadmium in Male Guinea Pigs (Cavia porcellus)

Brice Menkem; Bertin Narcisse Vemo; Megnimeza Martine Astride Tsambou; Tadiesse Lavoisier Fonou; Nguedia Arius Baulland Dongmo; Judith Laure Boufack; Margaret Mary Momo Chongsi; Augustave Kenfack

doi:10.3389/fvets.2021.736836

Therapeutic Effects of Ethanolic Extract of Polygonum limbatum meism Against Reproductive Toxicity Induced by Cadmium in Male Guinea Pigs (Cavia porcellus)

Front Vet Sci. 2021 Oct 12:8:736836. doi: 10.3389/fvets.2021.736836. eCollection 2021.

Authors

Brice Menkem¹, Bertin Narcisse Vemo², Megnimeza Martine Astride Tsambou¹, Tadiesse Lavoisier Fonou¹, Nguedia Arius Baulland Dongmo¹, Judith Laure Boufack¹, Margaret Mary Momo Chongsi¹, Augustave Kenfack¹

Affiliations

¹ Department of Animal Science, Faculty of Agronomy and Agricultural Sciences, University of Dschang, Dschang, Cameroon.
² Department of Animal Science, Faculty of Agriculture and Veterinary Medicine, University of Buea, Buea, Cameroon.

Abstract

This study aimed at evaluating the therapeutic effects of ethanolic extract of Polygonum limbatum meism (EEPLM) on the reproductive parameters of male Guinea pigs exposed to cadmium chloride. Thirty-six male guinea pigs were randomly assigned to six treatment groups (with six animals per group). Group 1 (DW) received distilled water orally; group 2 (Cd), negative control, was treated with cadmium chloride at a dose of 26.25 mg/kg body weight (bw); while group 3 (VitC), positive control, was given 26.25 mg of cadmium chloride/kg bw and 100 mg of vitamin C, and groups 4, 5, and 6 were treated, respectively, with EEPLM at doses of 50, 100, and 200 mg/kg bw in addition to cadmium chloride (26.25 mg/kg bw). After 90 days, all animals were sacrificed, and data related to reproduction, toxicity, and oxidative stress were collected. Results revealed a significant decrease (p < 0.05) of serum levels of creatinine, urea, alanine, and aspartate amino transferases in guinea pigs treated with cadmium chloride and EEPLM compared to the negative control group (Cd). The weight of the bulbo-urethral gland was significantly (p < 0.05) decreased in animals exposed to cadmium chloride and treated with vitamin C or EEPLM compared to the negative control (Cd). Guinea pigs orally receiving cadmium chloride and EEPLM showed significantly (p < 0.05) increased motility, sperm count, spermatozoa with entire plasma membrane, and percentage of normal spermatozoa with reference to the negative control (Cd). The serum level of testosterone increased insignificantly (p > 0.05) in animals given cadmium and EEPLM compared to the negative control (Cd). Animals co-administered cadmium chloride and EEPLM recorded a significantly (p < 0.05) reduced level of MDA, activities of SOD, and total peroxidases compared to the group that received cadmium chloride (Cd) only. In conclusion, cadmium chloride induced reproductive impairments by generating oxidative stress. However, the administration of EEPLM can mitigate these adverse effects due to its antioxidant properties.

Keywords: Polygonum limbatum meism; cadmium chloride; male guinea pigs; oxidative stress; reproduction.