Method development and validation for analysis of phenolic compounds in fatty complex matrices using enhanced matrix removal (EMR) lipid cleanup and UHPLC-QqQ-MS/MS

Zhiya Yin; Bo Yuan; Weiting Lyu; Qingrong Huang; James E Simon; Qingli Wu

doi:10.1016/j.foodchem.2021.131096

Method development and validation for analysis of phenolic compounds in fatty complex matrices using enhanced matrix removal (EMR) lipid cleanup and UHPLC-QqQ-MS/MS

Food Chem. 2022 Mar 30;373(Pt A):131096. doi: 10.1016/j.foodchem.2021.131096. Epub 2021 Oct 16.

Authors

Zhiya Yin¹, Bo Yuan¹, Weiting Lyu², Qingrong Huang³, James E Simon⁴, Qingli Wu⁵

Affiliations

¹ New Use Agriculture and Natural Plant Products Program, Department of Plant Biology Rutgers, The State University of New Jersey, 59 Dudley Road, New Brunswick, NJ 08901, USA; Department of Food Science, Rutgers, The State University of New Jersey, 65 Dudley Road, New Brunswick, NJ 08901, USA.
² New Use Agriculture and Natural Plant Products Program, Department of Plant Biology Rutgers, The State University of New Jersey, 59 Dudley Road, New Brunswick, NJ 08901, USA; Department of Medicinal Chemistry, Rutgers, The State University of New Jersey, 160 Frelinghuysen Road, Piscataway, NJ 08854, USA.
³ Department of Food Science, Rutgers, The State University of New Jersey, 65 Dudley Road, New Brunswick, NJ 08901, USA.
⁴ New Use Agriculture and Natural Plant Products Program, Department of Plant Biology Rutgers, The State University of New Jersey, 59 Dudley Road, New Brunswick, NJ 08901, USA; Department of Medicinal Chemistry, Rutgers, The State University of New Jersey, 160 Frelinghuysen Road, Piscataway, NJ 08854, USA; Center for Agricultureal Food Ecosystens, The New Jersey Institute for Food, Nutrition and Health, Rutgers University, 61 Dudley Road, New Brunswick, NJ 08901, USA. Electronic address: jimsimon@rutgers.edu.
⁵ New Use Agriculture and Natural Plant Products Program, Department of Plant Biology Rutgers, The State University of New Jersey, 59 Dudley Road, New Brunswick, NJ 08901, USA; Department of Food Science, Rutgers, The State University of New Jersey, 65 Dudley Road, New Brunswick, NJ 08901, USA; Department of Medicinal Chemistry, Rutgers, The State University of New Jersey, 160 Frelinghuysen Road, Piscataway, NJ 08854, USA; Center for Agricultureal Food Ecosystens, The New Jersey Institute for Food, Nutrition and Health, Rutgers University, 61 Dudley Road, New Brunswick, NJ 08901, USA. Electronic address: qlwu@sebs.rutgers.edu.

PMID: 34710678
DOI: 10.1016/j.foodchem.2021.131096

Abstract

Reliable analysis of phenolic compounds in fatty matrices is a challenging task. In this work, a robust analytical method was developed and validated for 55 phenolic compounds employing QuEChERS (quick, efficient, cheap, easy, rugged and safe) and Enhanced Matrix Removal (EMR)-lipid cleanup in 96-well plates for sample preparation, coupled with ultra-high performance liquid chromatography with triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS). Seven high-fat matrices of pork brain, belly and liver; horse serum, beef, salmon and avocado were explored for method validation and led to promising stepwise recoveries of extraction, clean-up, drying-reconstitution of most analytes ranging from 75% to 113%, and with an accuracy of 78%∼117%, except for six catechin-analogues. The matrix removal efficiency of EMR was determined using UHPLC-quadruple time of flight (QTOF)-MS, and results indicated that 56%∼77% of co-extractives were removed. This method would be readily extended to wide range of applications demanding high-throughput and sensitive analysis of phenolic compounds in fatty samples.

Keywords: Enhanced matrix removal; High-fat matrices; Phenolic compounds; QuEChERS; Solid phase extraction; UHPLC-QqQ-MS/MS.

MeSH terms

Animals
Cattle
Chromatography, High Pressure Liquid
Lipids
Persea*
Phenols
Solid Phase Extraction
Tandem Mass Spectrometry*

Substances

Lipids
Phenols