In Vitro Generation of Human Antibody-Secreting Cells Through the Stimulation of PBMCs with Dengue Virus Particles

Vivian Bonezi; Allan Henrique Depieri Cataneo; Pryscilla Fanini Wowk; Eduardo L V Silveira

doi:10.1007/978-1-0716-1879-0_16

In Vitro Generation of Human Antibody-Secreting Cells Through the Stimulation of PBMCs with Dengue Virus Particles

Methods Mol Biol. 2022:2409:235-244. doi: 10.1007/978-1-0716-1879-0_16.

Authors

Vivian Bonezi¹, Allan Henrique Depieri Cataneo², Pryscilla Fanini Wowk², Eduardo L V Silveira³

Affiliations

¹ Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil.
² Laboratório de Virologia Molecular, Instituto Carlos Chagas, Fundação Oswaldo Cruz, Curitiba, PR, Brazil.
³ Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil. eduardosilveira@usp.br.

PMID: 34709646
DOI: 10.1007/978-1-0716-1879-0_16

Abstract

The Dengue pathophysiology has had several aspects determined over the years. However, some points remain elusive, such as the metabolic factors that regulate the massive B cell differentiation into antibody-secreting cells observed in Dengue patients. In this chapter, we describe an in vitro method capable of mimicking this Dengue-induced cell expansion. More specifically, this approach allows dengue virus-stimulated peripheral blood mononuclear cells (PBMCs) from healthy individuals to enhance the frequency of phenotypical and functional antibody-secreting cells (ASCs) after 7 days of culture. A manuscript recently published by Bonezi and colleagues displays results generated through this methodology.

Keywords: Antibody-secreting cell differentiation; Cell culture; Dengue virus; PBMCs.

MeSH terms

Antibody-Producing Cells
Dengue Virus*
Dengue*
Humans
Leukocytes, Mononuclear
Virion