Roles of H2S and NO in regulating the antioxidant system of Vibrio alginolyticus under norfloxacin stress

Shuhe Chen; Yunsheng Chang; Yu Ding

doi:10.7717/peerj.12255

Roles of H₂S and NO in regulating the antioxidant system of Vibrio alginolyticus under norfloxacin stress

PeerJ. 2021 Oct 5:9:e12255. doi: 10.7717/peerj.12255. eCollection 2021.

Authors

Shuhe Chen^#¹, Yunsheng Chang^#¹, Yu Ding¹

Affiliation

¹ Fisheries College, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals and Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Guangdong Ocean University, Zhanjiang, Guangdong, China.

^# Contributed equally.

Abstract

Antioxidant system is of great importance for organisms to regulate the level of excessive reactive oxygen species (ROS) under the environmental stresses including antibiotics stress. Effects of norfloxacin (NOR) on cystathionine-β-synthase (CBS), nitric oxide synthase (NOS) and antioxidant enzymes were investigated, and interaction between NO and H₂S and their regulation on the antioxidant system of Vibrio alginolyticus under NOR were determined as well in the present study. After treated with 2 µg/mL NOR (1/2 MIC), CBS content, H₂S and NO contents decreased while H₂O₂ accumulation and the antioxidant-related genes mRNA level increased. Additionally, the endogenous H₂S content in V. alginolyticus was increased by the exogenous NO, while H₂O₂ accumulation and the relative expression level of SOD (Superoxide dismutase gene) decreased under exogenous NO or H₂S. And the content of endogenous NO and NOS in V. alginolyticus increased under the exogenous H₂S as well. Taken together, these results showed that anti-oxidative ability in V. alginolyticus was respectively enhanced by the gas molecules of H₂S and NO under NOR-induced stress, and there may be a crosstalk regulative mechanism between H₂S and NO. These results lay a foundation for the research of regulation network of H₂S and NO, and provide a hint to synthesize anti-vibrio drugs in the future.

Keywords: Antioxidant system; Cystathionine-β-synthase (CBS); H2S; NO; Nitric oxide synthetase (NOS); Norfloxacin; Vibrio alginolyticus.

Grants and funding

This work was supported by the grants from the Natural Science Foundation of Guangdong Province (No. 2014A030313604), the Graduate Education Innovation Program of Guangdong Ocean University (No. 201724) and the Foundation for the Distinguished Young Talents in Higher Education of Guangdong, China. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.