Identification and characterization of a novel endo-β-1,4-glucanase from a soil metagenomic library

Shumao Chai; Xueliang Zhang; Yuejiao Gao; Lishuang Nie; Chenchen Xiang; Mingming Cao; Shaochen Wang; Zhiyang Feng

doi:10.1016/j.carres.2021.108460

Identification and characterization of a novel endo-β-1,4-glucanase from a soil metagenomic library

Carbohydr Res. 2021 Dec:510:108460. doi: 10.1016/j.carres.2021.108460. Epub 2021 Oct 20.

Authors

Shumao Chai¹, Xueliang Zhang¹, Yuejiao Gao¹, Lishuang Nie¹, Chenchen Xiang¹, Mingming Cao¹, Shaochen Wang¹, Zhiyang Feng²

Affiliations

¹ College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China.
² College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China. Electronic address: zfeng@njau.edu.cn.

PMID: 34700218
DOI: 10.1016/j.carres.2021.108460

Abstract

A cosmid clone cZFYN1413 with CMCase activity was identified from a soil metagenomic library. The sequence analysis of a subclone of cZFYN1413 revealed an endo-β-1,4-glucanase gene ZFYN1413 belonging to glycoside hydrolase family 6 and a transmembrane region in the N-terminal of ZFYN1413. Expression of ZFYN1413 in Escherichia coli BL21 (DE3) resulted in ZFYN1413-87, which was a truncated protein cleaved in transmembrane region of ZFYN1413. ZFYN1413-87 was expressed and its enzyme properties were studied. ZFYN1413-87 possessed strong endo-β-1,4-glucanase activity, and 52% of the activity could be retained after the protein was treated in buffer of pH 3.0 for 2 h. The study provided a special example of endo-β-1,4-glucanase in GH6 family.

Keywords: Functional metagenomics; Glycoside hydrolase family 6; Transmembrane region; endo-β-1,4-Glucanase.

MeSH terms

Gene Library*
Glycoside Hydrolases / genetics*
Glycoside Hydrolases / metabolism
Metagenomics*
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Soil*

Substances

Recombinant Proteins
Soil
Glycoside Hydrolases